In Taiwan, most of female lung cancer patients are non-smoker (>90%) and most of the tumor types are adenocarcinoma. The previous studies indicated that cooking oil fumes, late menopausal ages and HPV infections have been associated with the risk of lung adenocarcinoma among non-smoking female. In order to discover novel molecular targets of non-smoking female lung cancer patients in Taiwan, the paired normal and tumor tissues were collected from 60 female non-smoking lung cancer patients for microarray analysis. From the microarray results, we found that several genes in the semaphorin family, such as SEMA3B, SEMA3G, SEMA5A, SEMA6A, and SEMA6D, were significantly down-regulated in the tumor tissues. However, the specific mechanisms of the genes in lung cancer are still unclear. Among the semaphorin genes, SEMA6A was chosen for further study. The results of quantitative real-time PCR and immunohistochemistry showed significant down-regulation of SEMA6A in lung cancer tissues. Moreover, the A549 cell line was used to further investigate the function of SEMA6A. A549 cells with overexpressed SEMA6A were identified and purified the cell membrane fraction for western blotting. A wound healing assay was also performed to address the migration ability. The result was shown that the A549 cells with over expressed SEMA6A exhibited a lower capacity to fill the gap during 24 hours incubation compared with A549 cells. In summary, our data indicated that normal lung tissues express more SEMA6A protein which might inhibit the cell migration than the cancer lung tissues.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1329.