SWAP-70 is a guanine nucleotide exchange factor (GEF) involved in membrane ruffling and functions through activation of Rac. In a gene expression profiling study comparing normal, low-grade and high-grade primary glioma specimens, SWAP-70 exhibited increasing expression with tumour grade. The subcellular localization of SWAP-70 was examined by immunofluorescence and we observed translocation of SWAP-70 to the membrane and ruffling following stimulation with EGF. The redistribution of SWAP-70 occurred without EGF stimulation in the SWAP-70 over-expressing cells. Motility and invasion assays showed increased migratory and invasive behavior following over-expression of SWAP-70. Conversely, membrane ruffling was decreased in SWAP-70 siRNA treated cells irrespective of EGF stimulation. In addition, down-regulation of SWAP-70 resulted in decreased motility and invasiveness as well as altered morphology of glioma cells. This study suggests that SWAP-70 functions in the motility of glial tumours. This is the first study to investigate a role for SWAP-70 in glioma cell morphology, motility, and invasiveness.

Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 2228.

100th AACR Annual Meeting-- Apr 18-22, 2009; Denver, CO