Introduction: Lung cancer is the second leading cause of cancer death in both men and women in the USA. The incidence is decreasing slightly in men, but rising sharply in women. Perhaps the greatest risk factor for developing lung cancer is exposure to inhalation carcinogens, most commonly those found in tobacco smoke. Other risk factors include asbestos and radon exposure. Despite improvements in therapy, the cure rate for lung cancer remains low. In general, surgery, radiation and chemotherapy are used in the management of lung cancer. But these treatments have adverse and toxic effects. Objective: We have developed strategies to inhibit cancer development and its spread using naturally occurring nutrients such as lysine, proline, ascorbic acid and green tea extract (NM). Such a unique formulation was shown to exhibit synergistic anticancer activity in vivo and in vitro in a number of cancer cell lines, inhibiting cancer cell growth, MMP secretion, invasion, metastasis and angiogenesis. The present study examines the in vivo effect of NM in the development of urethane-induced lung tumors in male A/J mice Methods: A/J male mice, six weeks old, were given a single intraperitoneal injection with urethane (1 mg/g body weight) in saline. After two weeks the mice were divided into two groups. Group 1 was fed a regular diet, while Group 2 was fed the same diet supplemented with 0.5% NM. After 20 weeks, mice were sacrificed, the lungs were excised and weighed, and tumors were counted and processed for histology. Results: There was no significant difference in the body weight of the control and test groups. Mice in both the groups developed tumors; however the numbers of tumors in the NM (Group 2) mice were significantly reduced by 50% compared to the control (Group 1) mice. Moreover lung weights of animals from the test group were less than in the control group. Pulmonary lesions were morphologically similar for both the groups (adenomas), but lesions were smaller in the test group. Conclusions: These results suggest that NM has inhibitory potential on the development of mouse lung tumors induced by urethane.
99th AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA