LPA stimulates cell migration, invasion and tumorigenesis of mouse ovarian cancer cells

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Ovarian cancer is the most deadly female reproductive disease. The major cause of death in women with ovarian cancer is from the spread of ovarian cancer cells to other organs and tissues (tumor metastasis) in the body. Cell migration and invasion are two common fundamental processes required for tumor metastasis. Lysophosphatidic acid (LPA), a bioactive lysophospholipid, regulates multiple important cellular functions, including the migration, invasion and adhesion of human ovarian cancer cells in vitro. In this study, we explored the role of LPA in a syngenic mouse model of ovarian cancer. We have shown for the first time that LPA accelerated tumorigenesis and/or metastasis, as well as promoted the formation of ascites in our immune-competent mouse model. In vitro, LPA promoted migration and invasion, but not the proliferation of the malignant, mouse ovarian surface epithelial cell (MOSEC) line, ID-8. Furthermore, AACOCF3, an inhibitor of cytosolic phosphalipase A₂ (cPLA₂), was found to be involved in both LPA-induced cell invasion and migration.