Purification of affinity tagged recombinant proteins is a critical first step for downstream functional and structural studies in many cancer research laboratories. Two commonly used affinity tags for the purification of recombinant proteins include the glutathione-S-transferase (GST) and poly-histidine (His) tags. An automated chromatography instrument has been developed for fast, reproducible, and cost-effective purification of both GST and His-tagged proteins. The system is configured for tandem chromatography applications and can perform time saving affinity purifications with integrated desalting (buffer exchange) in a single run. The system provides a complete set of chromatography buffers and affinity cartridges that have been optimized for GST and IMAC (immobilized metal affinity chromatography) separations. A user-friendly touch screen interface allows users to select pre-programmed chromatography methods or to customize methods for specific applications. The system is specifically designed for ease of use and for the unattended purification of 1-100 mg of GST or His-tagged proteins. Following purifications, instrument and column cleaning routines are performed to ensure optimal system performance. Representative purifications of GST tagged proteins using pre-programmed methods as well as custom methods designed to increase overall yield of purified protein are presented.

98th AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA