Study Purpose: The ginger root or rhizome (Zingiber officinale Roscoe, Zingiberaceae) is one of the most heavily consumed dietary substances in the world. Research has shown that ginger may prevent cancer and other chronic diseases most likely through anti-inflammatory and antioxidant properties. The purpose of this study was to develop and validate an HPLC assay to detect the main pungent ginger constituents (polyphenols made up of gingerols, shogaols, paradols and zingerone) that are most likely responsible for ginger root’s anti-inflammatory properties, in human plasma.

Methods: A method was developed to extract 6, 8 and 10-gingerols and 6-shogaol from 0.5 mL plasma samples with 50% ethyl acetate and 50% hexane (v/v) and pelargonic acid vanillylamide (PAV), a synthetic capsaicin, as the internal standard. Analysis was achieved using a reversed-phase C18 column with UV detection at 282 nm and electrochemical detection at 500, 550 and 600 µV.

Results: The method demonstrated linear performance from 0.05 to 5.0 µg/mL for all 4 ginger analytes in plasma. The coefficients of variation for intra- and inter-assays were ≤ 19.5% for 6-gingerol, ≤ 18.1% for 8-gingerol, ≤ 32.4% for 10-gingerol and ≤ 28.7% for 6-shogaol. The average recovery of 6-gingerol was > 81.3%, for 8-gingerol < 76.4%, for 10-gingerol < 98.2% and for 6-shogaol was < 73.5%.

Conclusions: This method provides a rapid and sensitive method for detecting 6, 8 and 10-gingerols and 6-shogaol in plasma.

98th AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA