Background/Aims: Recent years some results that some families of cancer testis antigens (CTAs) might be linked to chemotherapy-responses were reported. GAGE gene and Taxol resistance-associated gene 3 (TRAG-3) located on chromosome X were also hypothesized to be attributable to anticancer agent -resistant phenotypes. This study attempted to evaluate whether GAGE and TRAG-3 genes are involved in the drug-resistance phenotypes in colorectal cancers. Material and Methods: Surgical tumor and corresponding non-neoplastic fresh tissue samples from 60 patients with colorectal cancer provided with clinical and pathological characteristics were studied by RT-nested PCR. Results: The non-neoplastic colorectal mucosal tissues showed TRAG-3 with 11.7%(7/60) and GAGE expressions with 15%(9/60) of patients. Twenty of 60 (30%) showed TRAG-3 expression and 30 of 60 (50%) showed GAGE expression. Synchronous expressions of 2 genes was 15 of 60 patients.(25.0%). The expression of the GAGE and TRAG-3 genes were not related to age, sex, histological grades, the depth of invasion, metastasis to lymph nodes, vessel, neural, or perineural invasion and serum CEA level. There was no significant relationship of chemotherapy response to two each gene expressions. Conclusion: These results suggest that GAGE and TRAG-3 expressions might have a limited role in colorectal cancer without restriction to cancer tissues and not have the evidence on the association with the phenotype of chemotherapy resistance.
98th AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA