Multiple serum autoantibodies mini-array analysis on the subjects with esophageal precancerous and cancerous lesions from Linzhou, the highest incidence area for esophageal cancer in Henan, northern China Free

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The present study was designed to characterize the multiple autoantibodies of tumor associated antigens in sera from the patients in Linzhou, the highest incidence area for esophageal cancer (EC) in Henan, northern China to determine the possibility for high-risk subjects screening and early diagnosis for EC with these multi-tumor-associated antigens mini-array. Total 459 subjects were enrolled in this study, including 473 symptom-free subjects (265 males and 208 females with a mean age of 52±7) and 86 EC patients (48 males and 38 females with a mean age of 57±8). Five ml of fasting venous blood from each subject was collected into centrifuge tube in the field. The antigens for C-myc, cyclinB1, IMP1, Koc, p16, p53, p62 and Survivin were extracted and purified by American Scripps Research Institute. Indirect ELISA was used to detect the autoantibody in the serum samples. Each sample was repeated at least twice. ELISA results were evaluated based on the value of mean±3SD. The positive detection rate of autoantibodies for p53, C-myc, IMP1, p16 and p62 was higher than those of the 3 antigens and there was significant difference between normal and EC(P<0.05). In all grades of esophageal lesions, the positive rate of single antibody was low. However, the positive detection rate for EC increased 2~9 folds when the multiple positive markers were combined together for analysis. Comparison between autoantibodies in sera with matched antigens in different tissues showed that the positive rates of the 7 antigens (C-myc, cyclinB1, IMP1, Koc, p53, p62 and Survivin) except p16 in all grades of esophageal lesions and Survivin in NOR were much higher than that of autoantibodies in serum of matched lesions from the same patient(P<0.05). Though, there was no difference between the positive rate of p16 antibody in serum from patient with all grades of esophageal lesions and that of Survivin antibody in serum from normal subjects and that of matched antigen in tissue. However, the positive rate of these 2 antigens in these tissue was all 2~3 folds higher than that of antibodies in serum from the same type of patient. Small-scale clinical application with multiple sera autoantibodies for the high-risk subject screening and early diagnosis for SCC showed that the positive rate of esophageal basal cell hyperplasia, dysplasia and primary EC was 30%, 50% and 100%, respectively, which was confirmed by esophageal endoscopy and histopathology. The present results indicate that multiple serum autoantibodies mini-array analysis may be a promising method for high-risk subject screening and early cancer detection in EC. (supported by NNSFC 30025016 and 30670956).