Bladder cancer is the 4th and 8th most common cancer in men and women in the United sates, respectively. In an effort to identify genes upregulated in bladder tumors relative to normal bladder tissue, we have looked at Genelogic and GEPIS databases. Here we report a novel gene, named GOUTC, which is upregulated in urinary tract carcinomas and in bladder transitional cell carcinomas based on Genelogic database. The expression pattern of this gene is confirmed by real-time RT-PCR in clinical bladder tumor samples and bladder tumor cell lines. Overexpression of GOUTC in bladder cancer cell lines that express low level of GOUTC and grow relatively slowly, enhanced cell proliferation as evidenced by tritiated thymidine incorporation assay. The growth-promoting effects were not only seen in transient expressing cells, but also in stable transfectant cells. Furthermore, the cells stably expressing GOUTC promote anchorage-independent growth in soft agar assay. The stable cells formed not only more colonies but also larger colonies than the control cells. Using V5-tag, we found that the protein of GOUTC is in cytoplasm. Therefore, the GOUTC that we identified may represent a novel oncogenic molecule that promotes proliferation and anchorage-independent growth of bladder tumor cells or tumor progression.
[Proc Amer Assoc Cancer Res, Volume 47, 2006]