Through genome-wide cDNA microarray analysis coupled with microdissection of prostate cancer cells, we identified a novel gene, fatty acid-related molecule (FARM), as an over-expressed gene in prostate cancer cells. FARM is a highly conserved microsomal enzyme that is likely to be associated with fatty acid metabolism. Immunohistochemical analysis using polyclonal anti-FRAM antibody confirmed elevated expression of FARM protein in prostate cancer cells comparing to that in normal prostate epithelium or PINs. Knocking-down of FARM expression by siRNA in prostate cancer cell lines resulted in drastic attenuation of prostate cancer cell growth, suggesting that FARM expression could be essential to cell growth or viability of prostate cancer. A number of epidemiological or laboratory studies have been implicated that fatty acid metabolism could play some important roles in development and/or progression of prostate cancer. However, very little is known about the mechanism how fatty acids function in prostate cancer cells. Our findings demonstrate that a new member of fatty acid metabolic enzyme, FARM, is involved in growth and survival of prostate cancer cells thorough the metabolism of certain fatty acids and their derivatives, and that this molecule should be a promising molecular target for development of new therapeutic or preventive strategies for prostate cancers.
[Proc Amer Assoc Cancer Res, Volume 47, 2006]