Introduction: Current treatment for Burkitt’s lymphoma (BL) utilizes combined cytotoxic chemotherapy. But this treatment is highly toxic and is markedly less successful in AIDS-related BL. Therefore, additional therapy is urgently required. We previously demonstrated that a unique non-toxic nutrient mixture (NM) consisting of lysine, proline, ascorbic acid and green tea extract has antineoplastic activity against a number of cancer cell lines both in vitro and in vivo. Objective: In the present study we investigated the antineoplastic activity of NM on human Burkitt’s lymphoma Raji cells, evaluating cell proliferation, MMP expression and invasion through Matrigel. Method and Materials: Human Burkitt’s lymphoma Raji cells (ATCC) were grown in RPMI-1640 medium with 10% fetal bovine serum and antibiotics and treated with NM at 0, 10, 50, 100, 500 and 1000 μg/ml concentration in triplicate at each dose. Cell proliferation was assessed by counting cells stained with Trypan blue, invasion was evaluated through Matrigel, and MMP activity by gelatinase zymography. Cells were also treated to induce MMP-9 activity. Results: NM exhibited a dose response toxicity of 30%, 40%, 60%, and 90% at 10, 50, 100, 500 and 1000 μg/ml concentration respectively. Zymography demonstrated production of only MMP-9, which was further induced by PMA treatment. NM showed a significant decrease in MMP-9 expression at 500 μg/ml, which was totally abolished at 1000 μg/ml concentration. Invasion through Matrigel was inhibited at 10, 50 and 100 μg/ml by 60%, 85% and 100% respectively. Conclusions: Our results suggest that NM is a promising agent to be considered in the current treatment of BL in endemic and AIDS related diseases.
[Proc Amer Assoc Cancer Res, Volume 47, 2006]