In the article on human bone marrow–derived mesenchymal stem cells and gliomas in the April 15, 2005 issue of Cancer Research (1), Fig. 6C did not indicate that the middle figure was a montage created from five individual photomicrographs taken from regions between the stem cell injection site and the implanted tumor xenograft. For illustration purposes, the individual figures were postprocessed to highlight the red stem cells and the individual photomicrographs were placed on a black background, giving the incorrect impression that this was a single image. These data acquisition methods were not described in the figure legend or in Materials and Methods. Any manipulation of scientific illustrations is inconsistent with both previous journal standards and recently defined standards for electronic image collection, compilation, and alteration (2), and is not acceptable.

The authors have now corrected the figure to show the five individual photomicrographs, thereby eliminating the montage; the authors also have revised the figure legend. The new figure is free of any postprocessing, except where red and green images were merged with each other. This corrected version of the figure appears below. The authors note that the correction does not alter their conclusion that hMSCs are capable of migrating toward the tumor after intracranial injection.

Figure 6.

Photomicrographs showing migration of hMSCs toward glioma. U87 cells (gfp labeled) were implanted in the right frontal lobe (day 0) and SP-DiI-labeled hMSCs were implanted in the left lobe (day 7). Top, photomicrograph of brain section taken 14 days after cell injection. The boxes show the location of the images represented at the bottom. The numbers in the box correspond to the photomicrographs at the bottom. The corner where the number is located in the box at the top is the same corner of the corresponding photomicrograph at the bottom. Note the overlap of photomicrographs 1, 2, 4, and 5. Bottom, individual photomicrographs taken under the fluorescent microscope from regions shown on the top. Images 1 to 3, 4R, and 5R were taken with the red fluorescent filter. Images 4G and 5G were taken with the green filter. No green tumor cells were seen in the left brain (not imaged). 4R, 4G, 5R, and 5G were merged using PhotoShop to produce images 4M and 5M, respectively. Specifically, the corresponding red and green images were merged by copying the red image onto the green image and then using the Layer Style function and adjusting the Change Blend option, thus producing a merged picture. Red-fluorescent hMSCs are seen around the injection site (1), in between the injection site (2 and 3), and within the green-fsfluorescent tumor (4R, 4G, 4M, 5R, 5G, and 5M).

Figure 6.

Photomicrographs showing migration of hMSCs toward glioma. U87 cells (gfp labeled) were implanted in the right frontal lobe (day 0) and SP-DiI-labeled hMSCs were implanted in the left lobe (day 7). Top, photomicrograph of brain section taken 14 days after cell injection. The boxes show the location of the images represented at the bottom. The numbers in the box correspond to the photomicrographs at the bottom. The corner where the number is located in the box at the top is the same corner of the corresponding photomicrograph at the bottom. Note the overlap of photomicrographs 1, 2, 4, and 5. Bottom, individual photomicrographs taken under the fluorescent microscope from regions shown on the top. Images 1 to 3, 4R, and 5R were taken with the red fluorescent filter. Images 4G and 5G were taken with the green filter. No green tumor cells were seen in the left brain (not imaged). 4R, 4G, 5R, and 5G were merged using PhotoShop to produce images 4M and 5M, respectively. Specifically, the corresponding red and green images were merged by copying the red image onto the green image and then using the Layer Style function and adjusting the Change Blend option, thus producing a merged picture. Red-fluorescent hMSCs are seen around the injection site (1), in between the injection site (2 and 3), and within the green-fsfluorescent tumor (4R, 4G, 4M, 5R, 5G, and 5M).

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1
Nakamizo A, Marini F, Amano T, Khan A, Studeny M, Gumin J, Chen J, Hentschel S, Vecil G, Dembinski J, Andreef M, Lang FF. Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas.
Cancer Res
2005
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65
:
3307
–18.
2
Editorial. Nature 439, 891–892 (23 February 2006).