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Tumor necrosis factor related apoptosis-inducing ligand (TRAIL) is a TNF superfamily member that could induce apoptosis in wide range of human cell lines included glioma cells. In this study, oligonucleotide microarray that contained 20k human genes was used to analyze the change of gene expression profile in Glioblastoma cells compared between control and treatment with TRAIL in early responses. Results: Over 300 and 500 genes were up- and down-regulated with or more than four folds respectively. Up-regulated genes included tumor suppressor (e.g. DAB2, MKPX), cell cycle control (e.g. CDK4, CDK9) and apoptosis related (e.g. Caspase 2 and 10). Genes were down regulated included apoptosis inhibitors (Decoy receptor 1, 2 and 3) and chemokines (e.g. SCYB6, CXCL11). TRAIL receptor associated proteolysis protein-caspase 10 and an apoptosis inhibitor BIRC-2, were up regulated in 4 and 50 folds respectively. While the key executioner protein in apoptosis-caspase 3 remaining unchanged. Which may imply that TRAIL induced apoptosis could be rescued by up-regulating BIRC-2 to provide inhibitory effects. Further study of the pre-apoptotic responses will improve the understanding between human glioma cells and TRAIL.

[Proc Amer Assoc Cancer Res, Volume 46, 2005]