A striking feature of Ewing sarcoma is the presence of blood lakes lined by tumor cells. The significance of these structures, if any, is unknown. Here, we report that the extent of blood lakes correlates with poor clinical outcomes, whereas variables of angiogenesis do not. We also show that Ewing sarcoma cells form vessel-like tubes in vitro and express genes associated with vasculogenic mimicry. In tumor models, we show that there is blood flow through the blood lakes, suggesting that these structures in Ewing sarcoma contribute to the circulation. Furthermore, we present evidence that reduced oxygen tension may be instrumental in tube formation by plastic tumor cells. The abundant presence of these vasculogenic structures, in contrast to other tumor types, makes Ewing sarcoma the ideal model system to study these phenomena. The results suggest that optimal tumor treatment may require targeting of these structures in combination with prevention of angiogenesis. (Cancer Res 2005; 65(24): 11520-8)

Ewing sarcoma/primitive neuroectodermal tumor has been defined as a round cell sarcoma showing different degrees of neuroectodermal differentiation that present mostly in the bone or soft tissues (1). Ewing sarcoma is a relatively rare highly aggressive neoplasm with clinically evident metastatic disease at presentation in ∼25% of all patients. A striking feature of Ewing sarcoma, which often occurs at adolescence (24), is the presence of lakes of RBC. This was first recognized by James Ewing, which led him to regard the tumor as an endothelioma (5). Furthermore, Ewing sarcoma is characterized by a translocation that involves the Fli-1 gene, usually expressed specifically by endothelial cells. Prompted by these striking features, we considered that these tumors could provide unusual insight into the relationship between a growing solid tumor and the circulation.

When a growing tumor exceeds the size of ∼1 mm3, diffusion fails to provide the essential nutrients for continuous growth. Angiogenesis, a well-established paradigm in tumors, is subsequently stimulated driven by tumor-derived cytokines, such as vascular endothelial growth factors (VEGF) and fibroblast growth factors. Microvessels lined by endothelium grow into the tumor, providing a blood supply and allowing the tumor to grow beyond the limits imposed by substrate diffusion. It is observed in many tumor types that the extent of the capillary network, as assessed by the density of microvessels, is associated with poor prognosis (6, 7). There is considerable interest in the therapeutic potential of targeting the growth of new vessels (antiangiogenesis) and the patency of those that have been formed (vascular targeting).

Patients with Ewing sarcoma have previously been reported to have high levels of circulating VEGF (8). In view of this and the relatively poor prognosis of these tumors, we anticipated that the microvessel density would be high, supported by dynamic contrast enhanced magnetic resonance imaging studies (9). Surprisingly, we actually observed a relatively low microvessel density. This observation, together with the fact that the tumors contain blood lakes, led us to investigate the microcirculation of these tumors. We showed plasticity of tumor cells, using a combination of approaches, which we interpret as vasculogenic mimicry.

Vasculogenic mimicry was initially recognized in aggressive melanomas in 1999 (10), as a process in which tumor cells gain characteristics normally restricted to endothelial cells. Through this means, tumor cells could contribute to conducting blood in vascular-like structures, a process that would be independent of regular angiogenesis and endothelial cell proliferation. After these initial observations in melanoma, evidence for vasculogenic mimicry has been reported in other tumors (1114). However, the mechanisms driving the vasculogenic mimicry process, and the contribution of the tumor cell channels to the circulation, have been uncertain.

Patient tissue materials. Tumor tissues from 33 patients from the University of Leuven and the Leiden University Medical Center were studied, who presented with Ewing sarcoma between 1987 and 2004. Most patients were included in the European Intergroup Cooperative Ewing's Sarcoma Study and EuroEwing studies (15). Mean age was 23.8 years at diagnosis (range: 3-59 years). Patient data were available from 31 patients; 11 died before the moment of data analysis. Mean follow-up was 50 months and mean survival was 34 months. Twenty patients of the 23 analyzed had an 11;22 translocation. All patient materials were handled in a coded fashion according to the protocols as detailed by the Dutch association of Medical Scientific Associations.

Immunohistochemistry. Paraffin sections were dried for 48 hours at 37°C before staining. Histochemical staining included H&E staining and periodic acid Schiff's (PAS) reagent staining. The amount of blood lakes and PAS-positive loops was semiquantitatively assessed by scoring 0-4 (0, absent; 1, <5%; 2, 5-20%; 3, 20-50%; 4, >50% of tissue area). For immunohistochemistry, primary antibodies used were CD31 (1:50 dilution, DAKOCytomation, Glostrup, Denmark), CD34 (1:50, Novocastra, Valkenswaard, the Netherlands), Ki-67 (1:100, Labvision, Fremont, CA), VEGF (1:100 dilution, Santa Cruz, Tebu Bio, Heerhugowaard, the Netherlands), endoglin/CD105 (1:50 dilution, Monosan, Uden, the Netherlands), VE-cadherin (1:50, Cayman, Ann Arbor, MI), and tissue factor pathway inhibitor (TFPI, 1:40, American Diagnostica, Inc., Stamford, CT). After washing, the sections were incubated with antimouse immunoglobulin (1:200, DAKOCytomation) or antirabbit immunoglobulin biotin-labeled secondary antibody (1:200, DAKOCytomation) followed by avidin/biotin-horseradish peroxidase (DAKOCytomation) and 3,3′-diaminobenzidine as substrate. For dual staining, sections were first labeled for Ki-67 using horseradish peroxidase and then CD31 and CD34 using alkaline phosphatase reaction (DAKOCytomation). Microvessel density, proliferating tumor cells, and proliferating endothelial cells were assessed by counting in quadruplicate randomly chosen fields at 200× magnification (0.25 mm2) by three independent observers. Microvessel density was assessed in Ewing sarcoma tissues and also in 117 colorectal carcinomas, 211 renal cell carcinomas, 121 breast carcinomas, and 78 melanomas.

Cell culture and in vitro three-dimensional tube formation. Melanoma cell lines MUM-2B, MUM-2C, C8161, and C81-61 were used. Ewing sarcoma cell lines EW-7, A673, RD-ES, and SIM/EW27 were previously characterized by Dr. O. Delattre, RD-ES was obtained from American Type Culture Collection (Manassas, VA). Cells were maintained in RPMI 1640, supplemented with 10% FCS and 2 mmol/L glutamine, except for C81-61 (HAM F-10) and A673 (DMEM; Life Technologies, Paisley, United Kingdom). Cells grew on standard culture dishes except for the SIM-EW27, which were grown on collagen-coated culture dishes. Human umbilical vein–derived endothelial cells were cultured on gelatin-coated culture dishes in RPMI 1640, supplemented with 20% human serum, 100 units/mL penicillin, and 0.1 mg/mL streptomycin. For the three-dimensional culture, cells were plated at 100,000 cells per well on 24-well dishes coated with rat tail collagen, as described previously (10), and cultured for 10 days.

Vasculogenic tube formation was tested using a commercial Matrigel assay kit (BD Biocoat, Woerden, the Netherlands). Cells were plated at 20,000 cells per well and grown for 16 hours before calcein labeling and fluorescence microscopy. The effect of VEGF at 1, 10, and 50 ng/mL (Peprotech, Rocky Hill, NJ) or VEGF blocking antibody HuMV833 (1:50 dilution, Protein Design Labs, Inc., Fremont, CA) was assessed on Matrigel for 16 hours followed by counting the number of branch points per microscopic field.

RNA isolation and reverse transcription-PCR analysis. Total RNA was isolated from tissue sections using RNeasy (Qiagen, Venlo, the Netherlands), followed by RNase-free DNase treatment (Qiagen, Hilden, Germany). RNA concentration was measured by nanodrop (Nanodrop, Wilmington, DE). cDNA was synthesized with 400 units of M-MLV RNase H-reverse transcriptase (Promega, Leiden, the Netherlands) in 20 μL of 1× first strand buffer (Promega), and 1 mmol/L deoxynucleotide triphosphates in the presence of 10 units RNase inhibitor RNasin (Promega), 0.5 μg random primers (Promega), and 1 μg total RNA. RNA from cell lines was isolated using TRIzol (Invitrogen, Breda, the Netherlands). Semiquantitative PCR amplifications were done with primer sequences listed in Table 1 with glyceraldehyde-3-phosphate dehydrogenase primers (BD Clontech, Mountain View, CA) as a control. PCR fragments were ligated into pCR2.1-TOPO (Invitrogen) and two independent clones were sequenced from each primer set and shown to be identical to the expected DNA sequence. Quantitative real-time reverse transcription-PCR (RT-PCR) was done for cyclophylin A, β-actin, VEGF-A, VEGF-C, VEGF-D, angiopoeitin-1, basic fibroblast growth factor, and placenta growth factor as described (16). All real-time PCR primers were synthesized by Sigma-Genosys (Cambridgeshire, United Kingdom) except for VE-cadherin (a FAM-TAMRA primer probe, Integrated DNA Technologies, Inc., Coralville, IA). PCR was done using an iCycler MyIQ (Bio-Rad, Veenendael, the Netherlands) in 25 μL volume containing 0.8 μL cDNA, 1×SYBR Green PCR master mix (Eurogentec, Liege, Belgium) added to 20 nmol/L fluorescein (Bio-Rad) and 400 nmol/L of each primer. Data was analyzed with the Sequence Detection System software (Applied Biosystems, Foster City, CA). Experiments were done twice in duplicate.

Table 1.

Primers used for semiquantitative and quantitative RT-PCR

GenePCR primers (forward, reverse)Amplicon size (bp)Cycle no.Real-time PCR primers (forward, reverse)
EphA2 5′-GTTGAAGTTCACTACCGAGATCCATCCATCC-3′, 5′-GAGCCGGATAGACACGCGG-3′ 883 30 5′-AGACGCTGAAAGCCGGCTAC-3′, 5′-CAGGGCCCCATTCTCCATG-3′ 
VE-cadherin 5′-CACCGGCGCCAAAAGAGAGA-3′, 5′-CTGGTTTTCCTTCAGCTGGAAGTGGT-3′ 1,032 30 5′-TCCCGGAGCAGAAGACGT-3′, 5′-GAGAAAAGAAAGAGAGCATGGATTG-3′, probe 6-FAM/TGCATGACGGAGCCGAGCCA/36-TAMRA 
TIE-1 5′-CCCTGAGCTACCCAGTGCTAGAGTG-3′, 5′-TGGTCACAGGTTAGACAGCAGAGTTTG-3′ 1,000 30 5′-CCCCGCTGGTCTCGTTCTC-3′, 5′-CACAATGGTCGACCAGTCC-3′ 
VEGF-C 5′-CAGGCAGCGAACAAGACCTGC-3′, 5′-CAGATGAGCTCCAGTCCATTTCTGTAAAG-3′ 721 30  
Laminin5 γ2 5′-TCAGCCAGAAGGTTTCAGATGCC-3′, 5′-GGCAGCTTCACTGTTGCTCAAGCAG-3′ 654 30 5′-GTTGATACCAGAGCCAAGAAC-3′, 5′-GAAAGCTTCTGCTCCAGTAAG-3′ 
PAX 8 5′-CGATGCCTCACAACTCCATCAGAT-3′, 5′-GCTCGCCTTTGGTGTGGCT-3′ 694 30  
Urokinase 5′-TGTGGCCAAAAGACTCTGAGGC-3′, 5′-CTTGGTGTGACTGCGGATCCA-3′ 771 27  
Keratin 7 5′-CCAGCGGGTGCGCCA-3′, 5′-CACTCCATCTCCAGCCAACCG-3′ 973 30 (5% DMSO added)  
CD13 5′-CTGCTCCAACGGAGTTCCAGAGTG-3′, 5′-CTCAGGCAGCCTGGGTCATCA-3′ 812 30  
Vimentin 5′-CCTGAACCTGAGGGAAACTAATCTGGATT-3′, 5′-CTAAATCTTGTAGGAGTGTCGGTTGTTAAGAACTAGAG-3′ 313 30 (5% DMSO added)  
c-met 5′-CCAAGTCAGATGTGTGGTCCT-3′, 5′-TCGTGTGTCCACCTCATCAT-3′ 362 27 (annealing temperature 62°C)  
GAPDH 5′-TGAAGGTCGGAGTCAACGGATTTGGT-3′, 5′-CATGTGGGCCATGAGGTCCACCAC-3′ 983 25  
TFPI-1    5′-TTTGTGSSGSTGGTCCGAAT-3′, 5′-AGACACCATGAGGGACCG-3′ 
TFPI-2    5′-AGTGTGGACGACCAGTGTGAGG-3′, 5′-TGCGCAGAAGCCCATACAAG-3′ 
Tie-2    5′-TTGAAGTGGAGAGAAGGTCTG-3′, 5′-GTTGACTCTAGCTCGGACCAC-3′ 
Neuropilin    5′-ATGACGACCAGGCCAACT-3′, 5′-AACTCTGATTGTATGGTGCTGT-3′ 
Endoglin    5′-GCCAGCATTGTCTCACTTCAT-3′, 5′-TACCAGGGTCATGGCGTC-3′ 
GenePCR primers (forward, reverse)Amplicon size (bp)Cycle no.Real-time PCR primers (forward, reverse)
EphA2 5′-GTTGAAGTTCACTACCGAGATCCATCCATCC-3′, 5′-GAGCCGGATAGACACGCGG-3′ 883 30 5′-AGACGCTGAAAGCCGGCTAC-3′, 5′-CAGGGCCCCATTCTCCATG-3′ 
VE-cadherin 5′-CACCGGCGCCAAAAGAGAGA-3′, 5′-CTGGTTTTCCTTCAGCTGGAAGTGGT-3′ 1,032 30 5′-TCCCGGAGCAGAAGACGT-3′, 5′-GAGAAAAGAAAGAGAGCATGGATTG-3′, probe 6-FAM/TGCATGACGGAGCCGAGCCA/36-TAMRA 
TIE-1 5′-CCCTGAGCTACCCAGTGCTAGAGTG-3′, 5′-TGGTCACAGGTTAGACAGCAGAGTTTG-3′ 1,000 30 5′-CCCCGCTGGTCTCGTTCTC-3′, 5′-CACAATGGTCGACCAGTCC-3′ 
VEGF-C 5′-CAGGCAGCGAACAAGACCTGC-3′, 5′-CAGATGAGCTCCAGTCCATTTCTGTAAAG-3′ 721 30  
Laminin5 γ2 5′-TCAGCCAGAAGGTTTCAGATGCC-3′, 5′-GGCAGCTTCACTGTTGCTCAAGCAG-3′ 654 30 5′-GTTGATACCAGAGCCAAGAAC-3′, 5′-GAAAGCTTCTGCTCCAGTAAG-3′ 
PAX 8 5′-CGATGCCTCACAACTCCATCAGAT-3′, 5′-GCTCGCCTTTGGTGTGGCT-3′ 694 30  
Urokinase 5′-TGTGGCCAAAAGACTCTGAGGC-3′, 5′-CTTGGTGTGACTGCGGATCCA-3′ 771 27  
Keratin 7 5′-CCAGCGGGTGCGCCA-3′, 5′-CACTCCATCTCCAGCCAACCG-3′ 973 30 (5% DMSO added)  
CD13 5′-CTGCTCCAACGGAGTTCCAGAGTG-3′, 5′-CTCAGGCAGCCTGGGTCATCA-3′ 812 30  
Vimentin 5′-CCTGAACCTGAGGGAAACTAATCTGGATT-3′, 5′-CTAAATCTTGTAGGAGTGTCGGTTGTTAAGAACTAGAG-3′ 313 30 (5% DMSO added)  
c-met 5′-CCAAGTCAGATGTGTGGTCCT-3′, 5′-TCGTGTGTCCACCTCATCAT-3′ 362 27 (annealing temperature 62°C)  
GAPDH 5′-TGAAGGTCGGAGTCAACGGATTTGGT-3′, 5′-CATGTGGGCCATGAGGTCCACCAC-3′ 983 25  
TFPI-1    5′-TTTGTGSSGSTGGTCCGAAT-3′, 5′-AGACACCATGAGGGACCG-3′ 
TFPI-2    5′-AGTGTGGACGACCAGTGTGAGG-3′, 5′-TGCGCAGAAGCCCATACAAG-3′ 
Tie-2    5′-TTGAAGTGGAGAGAAGGTCTG-3′, 5′-GTTGACTCTAGCTCGGACCAC-3′ 
Neuropilin    5′-ATGACGACCAGGCCAACT-3′, 5′-AACTCTGATTGTATGGTGCTGT-3′ 
Endoglin    5′-GCCAGCATTGTCTCACTTCAT-3′, 5′-TACCAGGGTCATGGCGTC-3′ 

Xenograft assays and intravital microscopy. All animal experiments were approved by the local animal ethics committee. EW7 cells (106) were injected s.c. into the flank of Swiss/nude mice and tumors measured with calipers. On day 28, mice were anesthetized with ketamine/xylazine (17) and intravital microscopy was done as described before (18). In two mice, FITC dextran (MW 500 kDa, 500 μg) was injected i.v. in the tail vein. In these mice, imaging was done before and after injection. Two other mice were given an i.p. injection with pimonidazole 60 minutes before sacrifice (2 mg in 200 μL; Natural Pharmacia International, Research Triangle Park, NC). Sections of xenografts were labeled with anti–von Willebrand factor (1:200, DAKOCytomation) and anti-CD31 antibody (1:250, PharMingen, BD Company, Woerden, the Netherlands). Pimonidazole was detected using FITC-labeled antipimonidazole antibody (1:50 dilution; Hypoxyprobe-1 Mab1; Chemicon International, Inc., Temecula, CA) and horseradish peroxidase–conjugated anti-FITC antibody (1:200 times dilution, clone 5D6.2; Chemicon International).

In some experiments, 200 μg antiendothelial antibody (MECA20, a kind gift from Dr. A. Duijvestijn, Department of Immunology, University Maastricht, Maastricht, the Netherlands; ref. 19) was administered i.v. followed after 15 minutes by perfusion with Chinese ink (Pelikan, Hanover, Germany). Sections from those xenografts were labeled with peroxidase-labeled goat anti-rat immunoglobulin and counterstained with hematoxylin.

Statistical analyses. Real-time RT-PCR data were statistically analyzed using Mann-Whitney U tests (using SPSS-10 software). P values <0.05 were considered statistically significant. Statistical significance of experimental variables to clinical outcome was assessed by Mann-Whitney U test to compare between groups and log rank test and a Cox regression test were done for survival analysis.

The amount of blood lakes in Ewing sarcoma correlates to clinical outcome. In a unique series of Ewing sarcoma tissue biopsy samples, selected from patients before radiation or chemotherapy was started, we observed that microvessel density in these tumors, assessed by labeling of endothelial cells (microvessel density), was relatively low compared with other tumors (Fig. 1A). This led us to hypothesize that the blood lakes we observed in 92% of these tumors might play an important role in the tumor circulation (Fig. 1B). Supporting this notion, the blood lakes were not associated with evidence of coagulation and/or local necrosis. The cells lining the blood lakes did not label with the endothelial markers, CD31 and CD34. However, they expressed CD99, a marker of Ewing sarcoma cells and light microscopic examination (Fig. 1B) and electron-microscopic examination (not shown) clearly showed that the lake-lining cells were tumor cells and not endothelial cells. The presence of tumor cell–lined blood lakes has been described as vasculogenic mimicry. Vasculogenic mimicry was originally recognized in melanoma as PAS-positive loops that contained RBC (10). Also, the presence of PAS-positive loops was examined in Ewing sarcoma, which revealed that such loops were present in 68% of the patients (Fig. 1C). Notably, all tumors with PAS-positive networks also contained blood lakes.

Figure 1.

Vasculogenic mimicry in Ewing sarcoma. A, microvessel density quantification in several tumor types. *, P < 0.05, compared with the other tumor types. B, Ewing sarcoma patient tumor tissue stained with CD31/CD34 and counterstained with H&E. *, nonendothelial cell–lined structures containing erythrocytes or blood lakes. C, PAS staining of Ewing sarcoma. Bar, 100 μm (B and C). Quantification of blood lakes (D, *P < 0.04) and PAS-positive loops (E) in relation with clinical outcome. dod, death of disease.

Figure 1.

Vasculogenic mimicry in Ewing sarcoma. A, microvessel density quantification in several tumor types. *, P < 0.05, compared with the other tumor types. B, Ewing sarcoma patient tumor tissue stained with CD31/CD34 and counterstained with H&E. *, nonendothelial cell–lined structures containing erythrocytes or blood lakes. C, PAS staining of Ewing sarcoma. Bar, 100 μm (B and C). Quantification of blood lakes (D, *P < 0.04) and PAS-positive loops (E) in relation with clinical outcome. dod, death of disease.

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To address the significance of the blood lakes, we examined whether clinical outcomes correlated with the number of blood lakes. Interestingly, the number of blood lakes, quantified as the percentage of tumor area containing lakes, was significantly higher in the patients that subsequently died (75% higher compared with the group of patients still alive at the time of data analysis, P < 0.05; Fig. 1D).

Ewing sarcoma tumors are angiogenic; lack of correlation to clinical outcome. In tumors, we observed a high level of proliferation (mean number of Ki-67-positive tumor cells 51.4%, SD ± 30.4%). Highly proliferative tumors usually exhibit a high level of angiogenic signaling. On the other hand, the low microvessel density that we observed in the tumors suggested that these tumors might have limited angiogenic potential. We, therefore, investigated angiogenesis using several approaches and found evidence that Ewing sarcoma is highly angiogenic. First, we found that 22% of CD31/CD34–positive vessels had one or more Ki-67-positive nuclei (Fig. 2A). Second, in all Ewing sarcoma tissues, we found high mRNA expression levels of the angiogenic growth factors VEGF-A, basic fibroblast growth factor, placenta growth factor, and angiopoietin-1 comparable with those found in other angiogenic tumors, such as breast carcinoma and fibrosarcoma (Fig. 2B). Third, we observed high VEGF protein expression in ∼100% of tumor cells (Fig. 2C), in line with high circulating VEGF serum levels in Ewing sarcoma patients (8). Fourth, the cell lines EW7 and RD.ES, which give rise to tumors with typical Ewing sarcoma morphology following injection into athymic mice (see also Fig. 4), express high levels of angiogenic growth factors in vitro (Fig. 2B). Intriguingly, in the patient tissues, neither microvessel density nor the number of proliferating endothelial cells showed significant association with clinical outcome (Fig. 2D and E).

Figure 2.

Ewing sarcoma is highly angiogenic. A, double staining of Ewing sarcoma tissue with CD31/34 (blue) and Ki-67 (brown), indicating high numbers of proliferating endothelial cells (arrows). Bar, 100 μm. B, mean relative expression (in 2−ΔCt, corrected for housekeeping gene β-actin) VEGF-A, basic fibroblast growth factor (bFGF), placenta growth factor (PlGF), and angiopoietin-1 (ang-1) expression in Ewing sarcoma, fibrosarcoma, breast cancer tissue, and Ewing sarcoma tumor cell lines EW7 and RD.ES as measured by quantitative real-time RT-PCR. C, VEGF expression in Ewing sarcoma (conjugate control in top right corner; inset, scale bar represents 100 μm). Angiogenesis variables microvessel density (MVD; D) and proliferating endothelial cells (EC; E) do not correlate with clinical outcome.

Figure 2.

Ewing sarcoma is highly angiogenic. A, double staining of Ewing sarcoma tissue with CD31/34 (blue) and Ki-67 (brown), indicating high numbers of proliferating endothelial cells (arrows). Bar, 100 μm. B, mean relative expression (in 2−ΔCt, corrected for housekeeping gene β-actin) VEGF-A, basic fibroblast growth factor (bFGF), placenta growth factor (PlGF), and angiopoietin-1 (ang-1) expression in Ewing sarcoma, fibrosarcoma, breast cancer tissue, and Ewing sarcoma tumor cell lines EW7 and RD.ES as measured by quantitative real-time RT-PCR. C, VEGF expression in Ewing sarcoma (conjugate control in top right corner; inset, scale bar represents 100 μm). Angiogenesis variables microvessel density (MVD; D) and proliferating endothelial cells (EC; E) do not correlate with clinical outcome.

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Ewing sarcoma cells form vasculogenic structures in vitro and express vasculogenic mimicry–related genes. To investigate the capacity of different Ewing sarcoma cell lines to display vasculogenic mimicry in vitro, we used three-dimensional collagen matrix tube formation assays and done direct comparisons with a range of melanoma cell lines. The aggressive EW7 cell line (known to have a high tumorigenicity in mice) efficiently formed a network of tubular structures (Fig. 3A) comparable with the melanoma cell lines MUM-2B and C8161, which are aggressive and display vasculogenic mimicry in vitro (data not shown; refs. 10, 20). The less aggressive A673 and RD.ES Ewing sarcoma cell lines (known to have a low tumorigenicity in mice) showed an intermediate activity, forming a few tubular structures (Fig. 3B and C), whereas SIM/EW27 cells formed hardly any structures at all (Fig. 3D) comparable with the nonaggressive and vasculogenic mimicry–negative melanoma cell lines MUM-2C and C81-61 (not shown). Similar results were found on Matrigel (Fig. 3E-H).

Figure 3.

Ewing sarcoma cells form tubular structures in vitro and express vasculogenic mimicry–specific genes. Ewing sarcoma cell lines EW7 (A), A673 (B), RD.ES (C), and SIM-EW27 (D) in three-dimensional culture on collagen type I matrix. EW7 cells (E), A673 Ewing sarcoma cells (F), MUM-2B aggressive melanoma cells (G), and MUM-2C nonaggressive melanoma cells (H) on a Matrigel three-dimensional matrix stained with calcein. I, gene expression measured by semiquantitative RT-PCR in the Ewing sarcoma cell lines compared with the vasculogenic mimicry–positive melanoma cell line MUM-2B and the vasculogenic mimicry–negative cell line MUM-2C. The housekeeping gene GAPDH was used to control for equal loading. Quantitative real-time RT-PCR confirmation of VE-cadherin (J), integrin α3 (K), and TFPI-1 (L) gene expression of VE-cadherin in melanoma and Ewing sarcoma cell lines. The Ct values are corrected for the expression of housekeeping genes. Statistical significance determined by Mann-Whitney test. M, staining of Ewing sarcoma tissue sections with TFPI, VE-cadherin, and EphA2 antibodies. Bar, 100 μm [A, E (for A-H), and M].

Figure 3.

Ewing sarcoma cells form tubular structures in vitro and express vasculogenic mimicry–specific genes. Ewing sarcoma cell lines EW7 (A), A673 (B), RD.ES (C), and SIM-EW27 (D) in three-dimensional culture on collagen type I matrix. EW7 cells (E), A673 Ewing sarcoma cells (F), MUM-2B aggressive melanoma cells (G), and MUM-2C nonaggressive melanoma cells (H) on a Matrigel three-dimensional matrix stained with calcein. I, gene expression measured by semiquantitative RT-PCR in the Ewing sarcoma cell lines compared with the vasculogenic mimicry–positive melanoma cell line MUM-2B and the vasculogenic mimicry–negative cell line MUM-2C. The housekeeping gene GAPDH was used to control for equal loading. Quantitative real-time RT-PCR confirmation of VE-cadherin (J), integrin α3 (K), and TFPI-1 (L) gene expression of VE-cadherin in melanoma and Ewing sarcoma cell lines. The Ct values are corrected for the expression of housekeeping genes. Statistical significance determined by Mann-Whitney test. M, staining of Ewing sarcoma tissue sections with TFPI, VE-cadherin, and EphA2 antibodies. Bar, 100 μm [A, E (for A-H), and M].

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Using semiquantitative RT-PCR, we found that genes associated with vasculogenic mimicry–related tumor cell plasticity in melanoma (20) were expressed in the EW7 Ewing sarcoma cell line (Fig. 3I). We went on to perform quantitative real-time RT-PCR and found that integrin α3, VE-cadherin, TFPI-1 (Fig. 3J-L), EphA2, laminin5γ2, Tie-1, neuropilin, and endoglin (data not shown) were all highly overexpressed in EW7 and melanoma cell lines MUM-2B and C8161. Expression was lower or absent in Ewing's cell lines A673, RD.ES, and SIM-EW27 and the nonaggressive melanoma cell lines MUM-2C and C81-61, consistent with the degree of vasculogenic mimicry in three-dimensional culture (Fig. 3J-L).

To address whether these genes are expressed in vivo in Ewing sarcoma, we used quantitative RT-PCR to assay TFPI-1, VE-cadherin, and EphA2 in frozen material from patients and found that all three were expressed at a high level. Furthermore, using immunohistochemistry, we observed that TFPI-1/2, VE-cadherin, and EphA2 protein was present in tumor cells lining the vasculogenic mimicry structures (Fig. 2M).

Blood lake structures in Ewing sarcoma are part of the circulation. To study the functionality of blood lake structures in vivo, EW7 tumors were grown s.c. in the flank of athymic mice. Tumors grew rapidly and we observed numerous lakes, especially in the outer rim of these tumors (Fig. 4A and B), a distribution that has been previously described in melanomas (21). The cells lining these lakes were negative for von Willebrand factor and CD31. We used several approaches to investigate whether blood was flowing through these lakes. First, mice carrying EW7 tumors were i.v. injected with MECA20, an antibody specifically recognizing mouse endothelial cells. After 15 minutes, mice were sacrificed and perfused with an India ink suspension. We found ink both in MECA20 staining vessels as well as in vascular structures negative for MECA20 and directly lined by tumor cells (Fig. 4C). Second, intravital microscopy was done. The vasculogenic structures were characterized by irregular profiles and slow blood flow (Fig. 4D, a video image can be viewed on www.fdg.unimaas.nl/AngiogenesisLab). Such vasculogenic structures were not present in LS174T colon carcinoma tumors. In a third approach, the connection of vasculogenic structures in EW7 tumors with the circulation was shown when fluorescence was visualized in the vasculogenic structures after i.v. injection of a FITC-conjugated dextran (Fig. 4E).

Figure 4.

Vasculogenic mimicry structures in Ewing sarcoma are part of the circulation. EW7 Ewing sarcoma xenograft tumor tissue stained with H&E (A, paraffin-embedded tissue) and CD31 (B, frozen tissue). C, EW7 tumor of a mouse sacrificed 10 minutes after injection of MECA-20 and India ink. Arrows, blood lakes (A-C). Bar, 100 μm (B). D, an intravital microscopic image of Ewing sarcoma (a video image can be viewed on www.fdg.unimaas.nl/AngiogenesisLab). E, intravital microscopic image of an Ewing sarcoma tumor n a mouse injected i.v. with FITC-dextran. Bar, 100 μm (B, valid for D and E).

Figure 4.

Vasculogenic mimicry structures in Ewing sarcoma are part of the circulation. EW7 Ewing sarcoma xenograft tumor tissue stained with H&E (A, paraffin-embedded tissue) and CD31 (B, frozen tissue). C, EW7 tumor of a mouse sacrificed 10 minutes after injection of MECA-20 and India ink. Arrows, blood lakes (A-C). Bar, 100 μm (B). D, an intravital microscopic image of Ewing sarcoma (a video image can be viewed on www.fdg.unimaas.nl/AngiogenesisLab). E, intravital microscopic image of an Ewing sarcoma tumor n a mouse injected i.v. with FITC-dextran. Bar, 100 μm (B, valid for D and E).

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Blood flow in vasculogenic structures is inefficient; role of hypoxia. Somewhat unexpectedly, the abundant presence of blood lakes and PAS-positive loops in the patient tissues coincided with high levels of VEGF (and other angiogenic growth factors) and ongoing angiogenesis (high numbers of proliferating endothelial cells). We, therefore, considered whether VEGF is instrumental in the induction of blood lakes and tubes by tumor cells. However, this seems unlikely because (a) VEGF did not augment tube formation of EW7 or MUM-2B cells in the in vitro tube formation assay as examined at concentrations of 1 to 50 ng/mL (Fig. 5F); (b) VEGF did not increase expression of genes involved in tube formation by tumor cells, neither in tube forming nor in non–tube-forming tumor cells (Fig. 5G); and (c) addition of a VEGF blocking antibody was also not able to block or inhibit in vitro tube formation by the tumor cells; (Fig. 5H). Results are shown for TFPI-1 (Fig. 5F), similar results were found for VEGF receptor-1 and VEGF receptor-2, Tie-1, Neuropilin-1, laminin 5γ2, and EphA2. Furthermore, when comparing the VEGF RNA expression levels between tumors with higher amounts of blood lakes to tumors with lower numbers of lakes, this did not yield a difference in expression (data not shown).

Figure 5.

Blood lakes and hypoxia. A, Ewing sarcoma H&E-stained tumor section. B to D, CD31, HIF1α, and GLUT1 stainings, respectively. Arrows, regular blood vessels. E, pimonidazole adduct formation around blood lakes in a Ewing sarcoma mouse tumor. F, VEGF regulation of tube formation by EW7 cells cultured on Matrigel. G, regulation of vasculogenic mimicry–associated gene (TFPI-1) expression in EW7 by incubation for 48 hours in 10 ng/mL VEGF, MUM-2B, and MUM-2C cells. H, incubation of EW7 cells on a three-dimensional matrix with blocking VEGF antibody as a positive control human umbilical vein–derived endothelial cells (HUVEC) were used. Regulation of expression of vasculogenic mimicry–associated genes laminin 5γ2 (I), Tie-1 (J), TFPI-1 (K), and EphA2 (L) by hypoxia in EW7, A673, and C8161 cells. *, P < 0.05. Bar, 50 μm.

Figure 5.

Blood lakes and hypoxia. A, Ewing sarcoma H&E-stained tumor section. B to D, CD31, HIF1α, and GLUT1 stainings, respectively. Arrows, regular blood vessels. E, pimonidazole adduct formation around blood lakes in a Ewing sarcoma mouse tumor. F, VEGF regulation of tube formation by EW7 cells cultured on Matrigel. G, regulation of vasculogenic mimicry–associated gene (TFPI-1) expression in EW7 by incubation for 48 hours in 10 ng/mL VEGF, MUM-2B, and MUM-2C cells. H, incubation of EW7 cells on a three-dimensional matrix with blocking VEGF antibody as a positive control human umbilical vein–derived endothelial cells (HUVEC) were used. Regulation of expression of vasculogenic mimicry–associated genes laminin 5γ2 (I), Tie-1 (J), TFPI-1 (K), and EphA2 (L) by hypoxia in EW7, A673, and C8161 cells. *, P < 0.05. Bar, 50 μm.

Close modal

Although we did not find a relationship between tube formation by tumor cells and VEGF, a factor that is regulated by hypoxia, we investigated the expression of hypoxia-inducible factor 1α (HIF1α). This is a transcription factor potently stabilized under hypoxic conditions, which acts as a master regulator of gene expression in response to changes in oxygen tension (2224). Interestingly, we found HIF1α protein around the blood lakes but not around CD31+ vessels (Fig. 5B and C). Further evidence of HIF1α activation was provided by the observation that the glucose transporter, GLUT1, another HIF1α target gene, was also highly expressed in these regions (Fig. 5D). Because HIF1α can be activated by other variables besides low oxygen, we injected pimonidazole in Ewing sarcoma tumor-bearing mice to assess local oxygenation. This nitroimidazole forms adducts with cellular proteins only when oxygen tension is reduced. Like HIF1α protein, pimonidazole adducts were observed around the blood lakes, consistent with the notion that these regions are hypoxic (Fig. 5E, the tumors from mice not injected with pimonidazole did not stain positive; data not shown). Taken together, these findings suggested that hypoxic activation of HIF1α might be involved in driving vasculogenic mimicry. To test this idea, the expression of the above-mentioned genes that are involved in vasculogenic mimicry was investigated in aggressive and nonaggressive tumor cell lines cultured under hypoxic and standard conditions. We found that culturing for 16 hours under hypoxia significantly increased the expression of laminin5γ2, EphA2, Tie-1, and TFPI-1 (for all these molecules P < 0.01) in all cell lines tested (Fig. 5I-L).

Despite the clinically aggressive behavior of Ewing sarcoma, a relatively low number of microvessels was observed in this tumor type compared with other malignancies. We showed using several techniques that this was not due to a low angiogenic potential. Instead, the high angiogenic potential was illustrated by the high numbers of proliferating endothelial cells (although total number of vessels was low) and the high expression level (both RNA and protein) of angiogenic growth factors in the patient tissues as well as in the cell lines. The low amount of regular blood vessels, together with the abundant presence of blood lakes and PAS-positive loops (vascular-like tube formation by tumor cells), suggested a contribution of the blood lakes to circulation that might be considered vasculogenic structures as described earlier (10, 12, 13). We, therefore, suggest that Ewing sarcoma tumor cells cooperate in the formation of a circulatory system such as has been described as vasculogenic mimicry in aggressive melanoma. Interestingly, whereas in most tumors vasculogenic mimicry is present in 10% to 40% of cases, Ewing sarcoma is the first tumor in which vasculogenic mimicry is so abundantly present (blood lakes in 92% of cases). In Ewing sarcoma, the amount of blood lakes and presence of PAS-positive loops, in contrast to the classic angiogenesis variables, correlated with survival, confirming that vasculogenic mimicry is an indicator of poor prognosis (10, 25).

The vascular-like tube formation by Ewing sarcoma tumor cells was confirmed in a three-dimensional culture system using human cell lines, such as EW7, grown on a collagen matrix. Aggressive EW7 Ewing sarcoma cells rapidly formed vascular-like tubes in this system. In addition, EW7 cells injected into athymic mice gave rise to tumors with Ewing sarcoma morphology and blood lakes in vivo.

Furthermore, based on the similarities with the gene expression profiles in vasculogenic melanoma cells (13, 26), it was suggested that tube formation in Ewing sarcoma was due to a similar plasticity and dedifferentiation of tumor cells, which gained an endothelial phenotype. We showed that the genes involved in this process, such as TFPIs, EphA-2, and VE-cadherin (10, 27), are also highly expressed in the patient tissues and aggressive vascular-like tube-forming EW7 cells. In contrast, less aggressive Ewing sarcoma cells did not or less efficiently form these tubes and do not overexpress these genes. It is interesting in this context that one of the diagnostic indicators for Ewing sarcoma is a translocation of chromosomes 11 and 22, which involves the Fli-1 gene on chromosome 11 and the EWS gene on chromosome 22 (28, 29). In diagnostic pathology, Fli-1 antibodies are used as markers for Ewing sarcoma and for endothelial cells or vascular tumors. The protein that is formed by the Fli-1 gene is usually specifically expressed by endothelial cells. This translocation might play a role in the tumor cell plasticity seen in Ewing sarcoma.

Critical proof for the contribution of nonendothelial cell–lined structures in circulation was the demonstration of blood flow in these structures. Using intravital microscopy and immunohistochemistry after injection of antiendothelium antibody and India ink, we were able to show blood flow in the nonendothelial cell–lined vascular structures. This blood flow was observed to be very slow, which urged us to study oxygenation in the tumor tissue. When assessing the Ewing sarcoma tissue samples for hypoxia, we found that the tumor cells surrounding the blood lakes did express HIF1α, a transcription factor known to play a role in the expression of VEGF, as well as GLUT1, indicative of inefficient oxygen delivery by these structures. Possible explanations for this would include the very slow blood flow through these channels, and/or the possibility that they act primarily as a circulatory system draining blood from the tumor.

It is likely that the hypoxia that is induced in these tumors has led to the high expression of VEGF (and maybe other angiogenic factors) and resultant endothelial cell proliferation as observed in the patient tissues. An important possibility is that vascular-like tube formation by tumor cells might be induced by VEGF or other angiogenic factors. In fact, tube formation has previously been reported to be enhanced by VEGF in vitro (30). In contrast, in our study, we were not able to show that VEGF enhances or induces vascular-like tube formation by tumor cells. Although this could reflect the difference in tumor models and assay systems, we were also not able to show differences in expression of the genes involved in tube formation in response to VEGF (Fig. 5). Therefore, we favor the view that in Ewing sarcoma, blood lake and PAS-loop formation is not induced or supported by VEGF. However, we did find an increased expression of genes involved in vasculogenic mimicry when cells were cultured under low oxygen tension. Thus, we anticipate that hypoxia via induction of HIF1α is able to enhance vasculogenic mimicry. Interestingly, both HIF1α and vasculogenic mimicry associated genes (i.e., EphA2 and laminin-5γ2) signal via the PI3/K pathway (31, 32). Furthermore, blockade of this signal transduction pathway blocks vasculogenic mimicry and the expression of the genes involved in this process (33). In addition, Tie-1 can be up-regulated by HIF1α (34).

The results of this study strongly suggest that plasticity of Ewing sarcoma tumors is associated with the contribution of tumor cells to contribute to circulation. This presumably explains, at least in part, why the microvessel density is unusually low for such an aggressive tumor. This scenario may have effect on the treatment of tumors with angiogenesis inhibitors that act directly on endothelial cells. As we have shown before, dedifferentiating tumor cells do not acquire sensitivity to angiogenesis inhibitors (35), suggesting that an antiangiogenesis protocol may lead to only a partial regression of the tumor. Because vascular-like tube formation is much less frequent and/or less well recognized in other tumor types, it is possible that there is an important relationship between these structure and response to therapy, which has been overlooked in trials with angiogenesis inhibitors. Up to now, no data are available on such trials in Ewing sarcoma patients. We suggest that it would be particularly informative to seek a relationship between the presence of vasculogenic structures and the response to antiangiogenesis therapy. Furthermore, an interesting possibility is that antiangiogenesis therapy may result in a selective growth advantage for cells exhibiting vasculogenic mimicry, allowing drug-induced resistance to occur.

It seems likely that angiogenesis therapy may be more effective when combined with other forms of cancer therapies to eradicate vasculogenic tube formation, explaining the good results of combination between antiangiogenesis therapy and conventional cancer therapies in (pre)clinical studies (3638). The Ewing sarcoma model with its abundant vasculogenic structures may be an ideal model to develop and test therapies designed to counteract vasculogenic tube formation by attacking tumor cells that take part in the formation of vascular lakes (e.g., through CD99-directed therapy; ref. 39).

Grant support: Institute of Growth and Development at the University and the University Hospital of Maastricht.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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