Tissue lysate arrays: A rapid and accurate screen for protein expression. Free

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Protein expression profiling is an integral component of gene product discovery and biomarker identification. Typically, immunoblotting experiments are performed to examine protein expression profiles; this can be a time consuming process. To facilitate the rapid screening of multiple organs, of both normal and cancerous origin, we have constructed a tissue lysate array. This array consists of spotted pooled tissue lysates obtained from 34 tissue samples (17 organs, tumor and normal tissue from each) and 4 mammalian cell lines. Utilizing this array, we investigated the expression levels of proteins from several cellular compartments, among these the plasma membrane, cytosol, mitochondria and nucleus. Examples of analyzed proteins include MnSOD, ERK1, EGF receptor, insulin receptor and androgen receptor. Results obtained from the tissue lysate array and immunoblotting experiments were compared; immunoblotting data supports quantitative results obtained by the tissue lysate array. Our results demonstrate the utility of the tissue lysate array and support the use of this array as a tool for the rapid, simultaneous screening of human tissue and cell line lysate samples.