FX Enzyme Controls the Adhesive Properties of CRC

In the article on how FX enzyme controls the adhesive properties of CRC in the September 15, 2004 issue of Cancer Research (1), some of the labeling in Figures 7 and 10 was incorrect. The corrected figures are below.

Fig. 7.

Expression of FX mRNA, FX protein and of sLe-a by SW620 cells stably transfected with FX siRNA. A, FX mRNA. Expression was determined by Northern blot analysis. Values represent the ratio between the signal of FX mRNA in the cells and the signal of 18S rRNA in the same cell sample. B, FX protein. Expression was determined as in Fig. 2A. C, sLe-a. Expression was determined as in Fig. 2B. A representative experiment (of three performed) is presented (360 cells = control cells; 205 cells = FX siRNA transfectants). M = mean fluorescence; %pos = % positive cells.

Fig. 7.

Expression of FX mRNA, FX protein and of sLe-a by SW620 cells stably transfected with FX siRNA. A, FX mRNA. Expression was determined by Northern blot analysis. Values represent the ratio between the signal of FX mRNA in the cells and the signal of 18S rRNA in the same cell sample. B, FX protein. Expression was determined as in Fig. 2A. C, sLe-a. Expression was determined as in Fig. 2B. A representative experiment (of three performed) is presented (360 cells = control cells; 205 cells = FX siRNA transfectants). M = mean fluorescence; %pos = % positive cells.

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Fig. 10.

Expression of fucosylated proteins by SW620 cells stably transfected with FX siRNA. Lysates of control (360) and FX siRNA (205) transfected cells were assayed for the expression of fucosylated proteins by Western blot analysis using horseradish peroxidase-conjugated Ulex europaeus agglutinin 1. Expression of FX protein in the two cell populations was determined as in Fig. 2A. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading control.

Fig. 10.

Expression of fucosylated proteins by SW620 cells stably transfected with FX siRNA. Lysates of control (360) and FX siRNA (205) transfected cells were assayed for the expression of fucosylated proteins by Western blot analysis using horseradish peroxidase-conjugated Ulex europaeus agglutinin 1. Expression of FX protein in the two cell populations was determined as in Fig. 2A. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading control.

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1. Zipin A, Israeli-Amit M, Meshel T, Sagi-Assif O, Yron I, Lifshitz V, Bacharach E, Smorodinsky NI, Many A, Czernilofsky PA, Morton DL, Witz IP: Tumor-microenvironment interactions: the fucose-generating FX enzyme controls adhesive properties of colorectal cancer cells. Cancer Res 2004;64:6571–8.