NF-κB in Squamous Cell Carcinoma

In the article on NF-κB in squamous cell carcinoma in the September 15, 2004 issue of Cancer Research (1), the entries in Tables 1 and 2 indicating NF-κB modulated genes should have been boldfaced. The corrected Tables 1 and 2 are reproduced below.

1. Loercher A, Lee TL, Ricker JL, Howard A, Geoghegen J, Chen Z, Sunwoo JB, Sitcheran R, Chuang EY, Mitchell JB, Baldwin AS Jr, Van Waes C: Nuclear factor-κB is an important modulator of the altered gene expression profile and malignant phenotype in squamous cell carcinoma. Cancer Res 2004;64:6511–23

Table 1

Selected list of genes increased with tumor progression

Function GeneSymbolClone IDNF-κB AssociationFold change
LY-2/KerLY-2/IkB-aM
Cell cycle/growth      
 Cyclin D1* Ccnd1 H3084D05 Target gene 3.351 −2.266 
 Cyclin D2* Ccnd2 H3152D01 Target gene 2.957 −2.05 
 Growth arrest specific 5* Gas5 H3113A12  6.304 −7.813 
 Milk fat globule-EGF factor 8* Mfge8 H3126F11 Target gene 2.594 −3.525 
 Protein phosphatase 3*† Ppp3cb H3065C08 Inhibitor of NF-κB 3.117 −2.231 
 Proliferating cell nuclear antigen* Pcna H3021F12 Target gene 5.775 −2.811 
Apoptosis      
 Baculoviral IAP repeat* Birc2 H3074A02 Target gene 9.154 −6.013 
 Bcl-2 related ovarian killer*† Bok1 H3081D02  1.994 −3.461 
 Immediate early response 3* Ier3 H3057B07 Target gene 2.865 −3.851 
 Transformation related protein*† Trp53 H3142D07 Target gene 2.9 −4.142 
 Uchrp* Uchrp IMAGE:605056  2.188 −4.353 
Inflammation/angiogenesis      
 Colony stimulating factor 1* Csf1 H3057D05 Target gene 14.639 −5.574 
 Complement component 3*† C3 H3054A08 Target gene 5.145 −8.303 
 FGF receptor* Fgfr4 IMAGE:406823 Target gene 1.982 −1.991 
 Gro 1 oncogene* Gro1 H3051F10 Target gene 12.394 −4.094 
 Histocompatibility 2-L* H2-L H3096A12 Target gene 2.342 −3.431 
 Histocompatibility 2-D* H2-D H3141B11 Target gene 2.968 −3.927 
 Interferon receptor* Ifnar H3118F09 Inhibitor of NF-κB 4.5 −3.054 
 Lymphocyte antigen complex* Ly6e H3027D05 Inducer of NF-κB 4.029 −2.613 
Metastasis      
 Integrin α 3*† Itga3 H3137A03 Inducer of NF-κB 15.597 −3.917 
 Laminin α 5*† Lama5 H3002G01 Target gene 2.272 −2.967 
 Laminin receptor 1 Lamr1 H3075G08  2.169 −1.619 
 Plasminogen activator, tissue Plat H3080H11 Target gene 2.667 −2.487 
 Procollagen type 5 α 2 Col5a2 H3156E09 Target gene 5.103 −2.094 
 Syndecan 1* Sdc1 H3013F05 Target gene 2.948 −2.034 
Metabolism      
 ATPase H+ transport*† Atp6b H3120H04 Inhibitor of NF-κB 2.247 −2.858 
 Branched chain ketoacid dehyd* Bckdk H3136B09  2.455 −3.042 
 Choline kinase*† Chk H3088E07 Inducer of NF-κB 6.662 −3.252 
 Cytochrome p450* Cyp1b1 J0216F07 Target gene 22.289 −4.286 
 Glutathione-S-transferase* Gstm1 H3133A06 Target gene 3.037 −3.061 
 Low density lipoprotein receptor* Ldlr H3014C04 Target gene 2.689 −3.226 
 Mannose-6-phosphate receptor† M6pr H3092C05 Inhibitor of NF-κB 6.303 −3.612 
 Potassium intermediate* Kcnn4 H3054H04  2.778 −2.197 
 Solute carrier family 12*† Slc12a2 H3077B02  2.551 −2.145 
Stress response      
 Heat shock protein, 70 kDa* Hspa5 H3032A08 Activates NF-κB 14.303 −6.369 
 Heat shock protein 84 kDa* Hsp84 H3042G07 Activates NF-κB 3.437 −7.198 
 Heat shock protein 86 kDa* Hsp86 H3023G01 Activates NF-κB 2.61 −2.34 
 Heat shock protein cognate 70* Hsc70 H3133H01 Binds NF-κB 3.42 −10.229 
 Superoxide dismutase* Sod1 H3130B11 Target gene 4.784 −2.145 
Signal transduction      
 AXL receptortyrosine kinase*† Axl H3152F05 Inhibitor of NF-κB 2.459 0.938 
 CD97 (EGF-TM7)*† Cd97 H3032G06  2.283 −1.352 
 Interleukin-1 receptor associated* Il1rak H3042E08 Activates NF-κB 1.999 −1.296 
 Frizzled 7 homolog Fzd7 H3031A03  2.717 −0.843 
 Growth arrest & DNA damage specific* Gadd45g H3054C02 Activates NF-κB 3.144 −1.407 
 Growth factor receptor bound* Grb2 H3153D02 Activates NF-κB 2.483 −2.341 
 N-myc downstream regulated* Ndr2 G0110H06 Inhibits p50 2.248 −2.039 
 P13 kinase regulatory* Pik3r1 H3067B08 Activates NF-κB 4.206 −3.539 
 Protein tyrosine phosphatase*† Ptpn13 H3118G02 Inhibitor of NF-κB 2.844 −9.071 
 Ras p21 protein activator 3*† Rasa3 H3054E01 Activates NF-κB 3.223 −2.71 
 Ras-related C3* Rac1 IMAGE:477981  2.042 −3.875 
 Transferrin receptor* Trfr H3059G03  2.216 −1.145 
Nuclear proteins/transcription factors      
 Activating transcription factor† Atf2 J0221F08  1.606 −2.696 
 Breast cancer, early onset† Brca2 H3069F08  2.216 −0.604 
 Butyrate response factor* Brf2 H3015E08  2.072 −2.008 
 High mobility group AT* Hmga1 H3029B11 Target gene 2.816 −2.591 
 Jerky* Jrk H3119F06  2.849 −3.329 
 Myelocytomatosis oncogene* Myc H3089H11 Target gene 2.224 −2.42 
 Nuclear factor κB p105* Nfkb1 H3072E09  2.919 −1.443 
 Sex comb on midleg-like 1† Scml1 H3113B01  2.355 −1.154 
 Yes-associated protein 65 kDa Yap H3089H07  2.072 −1.746 
RNA processing      
 DEAD box protein 3* Ddx3 H3018F11  2.976 −2.055 
 DJ-1 protein† DJ-1 H3150D06  3.155 −4.611 
 FGF inducible 14 Fin14 H3018G01  2.358 −3.355 
 Nuclear ribonuclease Hnrpa1 H3111H11  4.926 −2.785 
 RNA polymerase 1-1* Rpo1-1 H3049D09  2.703 −2.328 
Protein synthesis/modification      
 ERO1 like*† Ero1l H3126B01  2.529 −2.456 
 Ribosomal protein L27a Rpl27a H3009B05  2.183 1.369 
Function GeneSymbolClone IDNF-κB AssociationFold change
LY-2/KerLY-2/IkB-aM
Cell cycle/growth      
 Cyclin D1* Ccnd1 H3084D05 Target gene 3.351 −2.266 
 Cyclin D2* Ccnd2 H3152D01 Target gene 2.957 −2.05 
 Growth arrest specific 5* Gas5 H3113A12  6.304 −7.813 
 Milk fat globule-EGF factor 8* Mfge8 H3126F11 Target gene 2.594 −3.525 
 Protein phosphatase 3*† Ppp3cb H3065C08 Inhibitor of NF-κB 3.117 −2.231 
 Proliferating cell nuclear antigen* Pcna H3021F12 Target gene 5.775 −2.811 
Apoptosis      
 Baculoviral IAP repeat* Birc2 H3074A02 Target gene 9.154 −6.013 
 Bcl-2 related ovarian killer*† Bok1 H3081D02  1.994 −3.461 
 Immediate early response 3* Ier3 H3057B07 Target gene 2.865 −3.851 
 Transformation related protein*† Trp53 H3142D07 Target gene 2.9 −4.142 
 Uchrp* Uchrp IMAGE:605056  2.188 −4.353 
Inflammation/angiogenesis      
 Colony stimulating factor 1* Csf1 H3057D05 Target gene 14.639 −5.574 
 Complement component 3*† C3 H3054A08 Target gene 5.145 −8.303 
 FGF receptor* Fgfr4 IMAGE:406823 Target gene 1.982 −1.991 
 Gro 1 oncogene* Gro1 H3051F10 Target gene 12.394 −4.094 
 Histocompatibility 2-L* H2-L H3096A12 Target gene 2.342 −3.431 
 Histocompatibility 2-D* H2-D H3141B11 Target gene 2.968 −3.927 
 Interferon receptor* Ifnar H3118F09 Inhibitor of NF-κB 4.5 −3.054 
 Lymphocyte antigen complex* Ly6e H3027D05 Inducer of NF-κB 4.029 −2.613 
Metastasis      
 Integrin α 3*† Itga3 H3137A03 Inducer of NF-κB 15.597 −3.917 
 Laminin α 5*† Lama5 H3002G01 Target gene 2.272 −2.967 
 Laminin receptor 1 Lamr1 H3075G08  2.169 −1.619 
 Plasminogen activator, tissue Plat H3080H11 Target gene 2.667 −2.487 
 Procollagen type 5 α 2 Col5a2 H3156E09 Target gene 5.103 −2.094 
 Syndecan 1* Sdc1 H3013F05 Target gene 2.948 −2.034 
Metabolism      
 ATPase H+ transport*† Atp6b H3120H04 Inhibitor of NF-κB 2.247 −2.858 
 Branched chain ketoacid dehyd* Bckdk H3136B09  2.455 −3.042 
 Choline kinase*† Chk H3088E07 Inducer of NF-κB 6.662 −3.252 
 Cytochrome p450* Cyp1b1 J0216F07 Target gene 22.289 −4.286 
 Glutathione-S-transferase* Gstm1 H3133A06 Target gene 3.037 −3.061 
 Low density lipoprotein receptor* Ldlr H3014C04 Target gene 2.689 −3.226 
 Mannose-6-phosphate receptor† M6pr H3092C05 Inhibitor of NF-κB 6.303 −3.612 
 Potassium intermediate* Kcnn4 H3054H04  2.778 −2.197 
 Solute carrier family 12*† Slc12a2 H3077B02  2.551 −2.145 
Stress response      
 Heat shock protein, 70 kDa* Hspa5 H3032A08 Activates NF-κB 14.303 −6.369 
 Heat shock protein 84 kDa* Hsp84 H3042G07 Activates NF-κB 3.437 −7.198 
 Heat shock protein 86 kDa* Hsp86 H3023G01 Activates NF-κB 2.61 −2.34 
 Heat shock protein cognate 70* Hsc70 H3133H01 Binds NF-κB 3.42 −10.229 
 Superoxide dismutase* Sod1 H3130B11 Target gene 4.784 −2.145 
Signal transduction      
 AXL receptortyrosine kinase*† Axl H3152F05 Inhibitor of NF-κB 2.459 0.938 
 CD97 (EGF-TM7)*† Cd97 H3032G06  2.283 −1.352 
 Interleukin-1 receptor associated* Il1rak H3042E08 Activates NF-κB 1.999 −1.296 
 Frizzled 7 homolog Fzd7 H3031A03  2.717 −0.843 
 Growth arrest & DNA damage specific* Gadd45g H3054C02 Activates NF-κB 3.144 −1.407 
 Growth factor receptor bound* Grb2 H3153D02 Activates NF-κB 2.483 −2.341 
 N-myc downstream regulated* Ndr2 G0110H06 Inhibits p50 2.248 −2.039 
 P13 kinase regulatory* Pik3r1 H3067B08 Activates NF-κB 4.206 −3.539 
 Protein tyrosine phosphatase*† Ptpn13 H3118G02 Inhibitor of NF-κB 2.844 −9.071 
 Ras p21 protein activator 3*† Rasa3 H3054E01 Activates NF-κB 3.223 −2.71 
 Ras-related C3* Rac1 IMAGE:477981  2.042 −3.875 
 Transferrin receptor* Trfr H3059G03  2.216 −1.145 
Nuclear proteins/transcription factors      
 Activating transcription factor† Atf2 J0221F08  1.606 −2.696 
 Breast cancer, early onset† Brca2 H3069F08  2.216 −0.604 
 Butyrate response factor* Brf2 H3015E08  2.072 −2.008 
 High mobility group AT* Hmga1 H3029B11 Target gene 2.816 −2.591 
 Jerky* Jrk H3119F06  2.849 −3.329 
 Myelocytomatosis oncogene* Myc H3089H11 Target gene 2.224 −2.42 
 Nuclear factor κB p105* Nfkb1 H3072E09  2.919 −1.443 
 Sex comb on midleg-like 1† Scml1 H3113B01  2.355 −1.154 
 Yes-associated protein 65 kDa Yap H3089H07  2.072 −1.746 
RNA processing      
 DEAD box protein 3* Ddx3 H3018F11  2.976 −2.055 
 DJ-1 protein† DJ-1 H3150D06  3.155 −4.611 
 FGF inducible 14 Fin14 H3018G01  2.358 −3.355 
 Nuclear ribonuclease Hnrpa1 H3111H11  4.926 −2.785 
 RNA polymerase 1-1* Rpo1-1 H3049D09  2.703 −2.328 
Protein synthesis/modification      
 ERO1 like*† Ero1l H3126B01  2.529 −2.456 
 Ribosomal protein L27a Rpl27a H3009B05  2.183 1.369 
View Large
Table 1A

Continued

Function GeneSymbolClone IDNF-κB AssociationFold change
LY-2/KerLY-2/IkB-aM
 Ribosomal protein L8 Rpl8 H3141F09  2.778 1.005 
 Ribosomal protein S18* Rps18 H3006C11  2.487 −2.424 
 Ubiquitin activating enzyme E1 Ube1x H3022E03 Phosphorylates κB 5.313 −3.139 
 Ubiquitin B Ubb H3138A08 Labels κB 8.144 −1.171 
 Ubiquitin conjugating enzyme E2 Ube2h H3057B09 Phosphorylates κB 3.333 −1.199 
 Ubiquitin conjugating enzyme E3† Ube3a H3102B01 Phosphorylates κB 4.878 −5.096 
 Ubiquitin specific protease 9† Usp9x H3139F12  3.769 −3.175 
Structural proteins      
 Alpha tropomyosin* Tpm1 H3120G06 Binds p65 2.421 −4.36 
 Cadherin* Cdh3 H3018F05 Inflammatory 2.328 −2.706 
 Capping protein α 2 Cappa2 H3085F12  14.293 −7.458 
 Dystroglycan 1* Dag1 H3008B05 Activates NF-κB 2.593 −4.738 
 Epithelial protein lost*† Eplin H3153C05 Activates NF-κB 2.066 −2.971 
 Fascin homolog 1* Fscn1 H3006D08  2.315 −2.581 
 Four and a half LIM domains* Fhl2 H3033C07  2.169 −1.189 
 Keratin complex 1 acidic* Krt1-18 H3021B02 Target gene 3.105 −5.517 
 Keratin complex 2 basic* Krt2-8 H3031C01  2.094 −5.568 
 PDZ and LIM domain 1* Pdlim1 J0824B03  5.7 −3.775 
 Protocadherin 7*† Pcdh7 H3067F12 Inflammatory 6.556 −1.026 
 Thymopoietin* Tmpo H3096B08  9.939 −8.153 
Other      
 Globin inducing factor† Gbif H3053F12  2.003 1.002 
 Metallothionein 2 Mt2 H3013D11 Inhibits IκB degradation 2.145 −3.029 
 Next to the Brca1*† Nbr1 H3061D04  2.367 −1.888 
 RAN binding protein* Ranbp9 H3013A10 Accumulates IκBα 2.87 −3.189 
 Repeat family 3 gene* Llrep3 H3107F07  3.556 −7.704 
 Ring finger protein 19* Rnf19 H3153A08 Activates NF-κB 2.148 −4.402 
 Sema domain, immunoglobin*† Sema3f H3134D09  2.191 −1.667 
 Suppressor of Lec15† Supl15h H3090D12  2.001 −1.383 
 TGF β inducible transcript* Tgfb1i1 H3122H01  3.068 −2.974 
Function GeneSymbolClone IDNF-κB AssociationFold change
LY-2/KerLY-2/IkB-aM
 Ribosomal protein L8 Rpl8 H3141F09  2.778 1.005 
 Ribosomal protein S18* Rps18 H3006C11  2.487 −2.424 
 Ubiquitin activating enzyme E1 Ube1x H3022E03 Phosphorylates κB 5.313 −3.139 
 Ubiquitin B Ubb H3138A08 Labels κB 8.144 −1.171 
 Ubiquitin conjugating enzyme E2 Ube2h H3057B09 Phosphorylates κB 3.333 −1.199 
 Ubiquitin conjugating enzyme E3† Ube3a H3102B01 Phosphorylates κB 4.878 −5.096 
 Ubiquitin specific protease 9† Usp9x H3139F12  3.769 −3.175 
Structural proteins      
 Alpha tropomyosin* Tpm1 H3120G06 Binds p65 2.421 −4.36 
 Cadherin* Cdh3 H3018F05 Inflammatory 2.328 −2.706 
 Capping protein α 2 Cappa2 H3085F12  14.293 −7.458 
 Dystroglycan 1* Dag1 H3008B05 Activates NF-κB 2.593 −4.738 
 Epithelial protein lost*† Eplin H3153C05 Activates NF-κB 2.066 −2.971 
 Fascin homolog 1* Fscn1 H3006D08  2.315 −2.581 
 Four and a half LIM domains* Fhl2 H3033C07  2.169 −1.189 
 Keratin complex 1 acidic* Krt1-18 H3021B02 Target gene 3.105 −5.517 
 Keratin complex 2 basic* Krt2-8 H3031C01  2.094 −5.568 
 PDZ and LIM domain 1* Pdlim1 J0824B03  5.7 −3.775 
 Protocadherin 7*† Pcdh7 H3067F12 Inflammatory 6.556 −1.026 
 Thymopoietin* Tmpo H3096B08  9.939 −8.153 
Other      
 Globin inducing factor† Gbif H3053F12  2.003 1.002 
 Metallothionein 2 Mt2 H3013D11 Inhibits IκB degradation 2.145 −3.029 
 Next to the Brca1*† Nbr1 H3061D04  2.367 −1.888 
 RAN binding protein* Ranbp9 H3013A10 Accumulates IκBα 2.87 −3.189 
 Repeat family 3 gene* Llrep3 H3107F07  3.556 −7.704 
 Ring finger protein 19* Rnf19 H3153A08 Activates NF-κB 2.148 −4.402 
 Sema domain, immunoglobin*† Sema3f H3134D09  2.191 −1.667 
 Suppressor of Lec15† Supl15h H3090D12  2.001 −1.383 
 TGF β inducible transcript* Tgfb1i1 H3122H01  3.068 −2.974 

NOTE. Total number of genes regulated by NF-κB = 105/167. Total number of genes previously associated with NF-κB = 67/167.

*

Genes containing κB site in promoter region.

†

Genes containing ACTACAG motif in coding sequence.

View Large
Table 2

Selected list of genes decreased with tumor progression

Function GeneSymbolClone IDNF-κB associationFold change
LY2/KerLY2/IkB-aM
Cell cycle/growth      
 C-src tyrosine kinase* Csk L0237H04  −2.693 2.358 
 Calmodulin Calm H3006H05 Activates NF-κB via IKK −2.554 2.42 
 Cell division cycle homolog 25a Cdc25a H3050E04  −2.341 2.269 
 Cell division cycle homolog 45 Cdc45l H3003E07  −2.032 2.029 
 Cyclin C Ccnc C0117F09  −2.739 2.418 
 Cyclin E2* Ccne2 C0186A01  −2.309 2.155 
 Cyclin dependent kinase 4 Cdk4 H3147D06 Target gene of NF-κB −2.734 2.05 
 Cyclin dependent kinase inhibit* Cdkn1c H3097D03  −5.208 2.913 
 Platelet derived growth factor* Pdgfa H3146C02  −2.15 2.906 
Apoptosis      
 ATP binding cassette* Abcd3 H3143E03 NF-κB site in promoter −2.89 2.535 
 Bcl2/adenovirus E1B Bnip3 H3103B07 Transient inhibitor of NF-κB −2.364 2.436 
 Fas associating w/death domain Fadd H3095D08 Inducer of NF-κB −3.597 2.975 
Inflammation/angiogenesis      
 Coagulation factor III*† F3 H3014G02 Activates NF-κB via IKK −4.672 3.858 
 Interleukin 17 receptor Il17r H3008A03 Activates NF-κB via MAPK −3.021 3.048 
 Interleukin 2 receptor Il2ra J0052C08 NF-κB site in promoter −2.262 2.207 
 Lymphocyte antigen 6 complex Ly6 H3115A08 Inducer of NF-κB −5.617 3.311 
 Prothymosin β Ptmb4 H3143A02  −21.739 8.858 
Metastasis      
 A disintegrin/MMP Adamts1 H3034B07  −2.695 2.736 
 Cadherin 1* Cdh1 H3076B06 Associated with inflammation −2.597 2.313 
 Kangai 1† Kai1 H3154D02 Target gene of NF-κB −2.816 2.139 
 Lipocalin 2 Lcn2 H3083G02 Associated with inflammation −2.506 11.235 
 Procollagen type 1 α Col1a2 H3125D01  −4.901 2.886 
 Procollagen type II α Col2a1 H3026G09  −6.896 2.139 
 Procollagen type III α Col3a1 H3005D11 Inducer of NF-κB −3.205 4.268 
 Secreted acidic C-rich Sparc H3026D08  −8.928 2.872 
 Tissue inhibitor of MMPs Timp3 H3031E01 Two NF-κB sites in promoter −3.3 3.289 
Metabolism      
 ATPase, type 11A† Atp11a H3097B05  −2.888 2.734 
 ATP synthase H+ transport Atp5j2 H3118C01 Inhibitor of NF-κB −2.191 2.111 
 Glutathione peroxidase* Gpx3 J0088G08 Inhibitor of NF-κB upregulates IkBa normal half-life −2.604 4.065 
 Lipopolysaccharide binding*† Lbp H3086G08 Activates NF-κB via MAPK −2.977 2.103 
 Phosphoprotein enriched† Pea15 H3014G07 Inducer of NF-κB −2.424 1.159 
 Pyruvate dehydrogenase*† Pdha1 H3068G07 NF-κB site in promoter −3.021 1.643 
 Sterol carrier protein 2*† Scp2 H3122F12  −2.412 1.488 
Stress response      
 Crystallin α 2 Crya2 H3143B04 Inhibitor NF-κB −3.921 3.479 
Signal transduction      
 Adenylate kinase† Ak2 H3052D11  −3.755 3.546 
 Max dimerization protein 4*† Mad4 H3131B07  −2.008 2.131 
 MAD homolog 4 Madh4 H3128C04  −2.765 2.114 
 NF-κB enhancer inhibitor* Nfkbia H3026A08  −1.433 4.611 
 NIK-related kinase Nrk H3008B02 Activates NF-κB −2.244 4.859 
 Phosphoglycerate kinase* Pgk1 H3023D06  −2.659 2.061 
 Protein tyrosine phosphatase 4 Ptp4a2 H3088F03  −2.118 1.615 
 Rho-associated coiled-coil Rock1 H3069C09  −2.808 3.183 
 TNF receptor associated factor Traf1 H3015E06 NF-κB dependent −2.011 1.243 
Nuclear proteins/transcription factors      
 Cbp/p300 interacting transactivation Cited4 H3076H08 NF-κB co-activator −2.178 2.745 
 High mobility group box 1 Hmgb1 H3126A05 Binds p50 subunit −3.104 1.739 
 Jun oncogene Jun H3058C09 NF-κB co-activator −2.906 2.057 
 Ras-related C3 Rac1 H3018C09 Inducer of NF-κB −2.004 3.329 
RNA processing      
 Nuclear protein 220† Np220 H3029A07  −2.259 1.827 
 RNA polymerase II* Rpo2-3 H3055H08 Coactivator of p65 −2.639 4.501 
Protein synthesis/modification      
 Eukaryotic translation 4g2 Eif4g2 H3113E10  −4.698 2.222 
 Nedd4 WW-binding protein 4* N4wbp4 H3062G06  −6.966 2.814 
Structural proteins      
 Alpha 2 glycoprotein 1*† Azgp1 IMAGE:521249  −2.013 1.398 
 Beta spectrin 2*† Spnb2 H3010G09  −3.781 1.015 
 Catenin beta* Catnb H3031E05 Regulated by IKK −3.998 3.061 
 Fibronectin† Fn1 H3116A10 NF-κB site in promoter −6.201 4.723 
 Catenin α 1*† Catna1 H3018E08  −1.996 1.723 
 Tenascin C Tnc L0062E01 NF-κB site in promoter −2.557 1.956 
Other      
 Deleted in polyposis Dp1 J0420H06  −2.473 2.137 
 Insulin-like growth factor r2 Igfr2 H3148G08  −2.765 2.494 
 Ninjurin 1* Ninj1 H3072B10  −2.579 4.878 
 Rabaptin 5*† Rab5ep H3002C01  −2.739 1.333 
 Topoisomerase II α*† Top2a H3139A05 Inducer of NF-κB −2.427 2.893 
 Tumor differentially expressed† Tde11 H3014H10  −2.593 1.18 
 WW domain binding 5* Wbp5 H3127H02  −2.087 3.313 
 Zinc finger protein 68*† Zfp68 H3058F07  −4.122 5.885 
Function GeneSymbolClone IDNF-κB associationFold change
LY2/KerLY2/IkB-aM
Cell cycle/growth      
 C-src tyrosine kinase* Csk L0237H04  −2.693 2.358 
 Calmodulin Calm H3006H05 Activates NF-κB via IKK −2.554 2.42 
 Cell division cycle homolog 25a Cdc25a H3050E04  −2.341 2.269 
 Cell division cycle homolog 45 Cdc45l H3003E07  −2.032 2.029 
 Cyclin C Ccnc C0117F09  −2.739 2.418 
 Cyclin E2* Ccne2 C0186A01  −2.309 2.155 
 Cyclin dependent kinase 4 Cdk4 H3147D06 Target gene of NF-κB −2.734 2.05 
 Cyclin dependent kinase inhibit* Cdkn1c H3097D03  −5.208 2.913 
 Platelet derived growth factor* Pdgfa H3146C02  −2.15 2.906 
Apoptosis      
 ATP binding cassette* Abcd3 H3143E03 NF-κB site in promoter −2.89 2.535 
 Bcl2/adenovirus E1B Bnip3 H3103B07 Transient inhibitor of NF-κB −2.364 2.436 
 Fas associating w/death domain Fadd H3095D08 Inducer of NF-κB −3.597 2.975 
Inflammation/angiogenesis      
 Coagulation factor III*† F3 H3014G02 Activates NF-κB via IKK −4.672 3.858 
 Interleukin 17 receptor Il17r H3008A03 Activates NF-κB via MAPK −3.021 3.048 
 Interleukin 2 receptor Il2ra J0052C08 NF-κB site in promoter −2.262 2.207 
 Lymphocyte antigen 6 complex Ly6 H3115A08 Inducer of NF-κB −5.617 3.311 
 Prothymosin β Ptmb4 H3143A02  −21.739 8.858 
Metastasis      
 A disintegrin/MMP Adamts1 H3034B07  −2.695 2.736 
 Cadherin 1* Cdh1 H3076B06 Associated with inflammation −2.597 2.313 
 Kangai 1† Kai1 H3154D02 Target gene of NF-κB −2.816 2.139 
 Lipocalin 2 Lcn2 H3083G02 Associated with inflammation −2.506 11.235 
 Procollagen type 1 α Col1a2 H3125D01  −4.901 2.886 
 Procollagen type II α Col2a1 H3026G09  −6.896 2.139 
 Procollagen type III α Col3a1 H3005D11 Inducer of NF-κB −3.205 4.268 
 Secreted acidic C-rich Sparc H3026D08  −8.928 2.872 
 Tissue inhibitor of MMPs Timp3 H3031E01 Two NF-κB sites in promoter −3.3 3.289 
Metabolism      
 ATPase, type 11A† Atp11a H3097B05  −2.888 2.734 
 ATP synthase H+ transport Atp5j2 H3118C01 Inhibitor of NF-κB −2.191 2.111 
 Glutathione peroxidase* Gpx3 J0088G08 Inhibitor of NF-κB upregulates IkBa normal half-life −2.604 4.065 
 Lipopolysaccharide binding*† Lbp H3086G08 Activates NF-κB via MAPK −2.977 2.103 
 Phosphoprotein enriched† Pea15 H3014G07 Inducer of NF-κB −2.424 1.159 
 Pyruvate dehydrogenase*† Pdha1 H3068G07 NF-κB site in promoter −3.021 1.643 
 Sterol carrier protein 2*† Scp2 H3122F12  −2.412 1.488 
Stress response      
 Crystallin α 2 Crya2 H3143B04 Inhibitor NF-κB −3.921 3.479 
Signal transduction      
 Adenylate kinase† Ak2 H3052D11  −3.755 3.546 
 Max dimerization protein 4*† Mad4 H3131B07  −2.008 2.131 
 MAD homolog 4 Madh4 H3128C04  −2.765 2.114 
 NF-κB enhancer inhibitor* Nfkbia H3026A08  −1.433 4.611 
 NIK-related kinase Nrk H3008B02 Activates NF-κB −2.244 4.859 
 Phosphoglycerate kinase* Pgk1 H3023D06  −2.659 2.061 
 Protein tyrosine phosphatase 4 Ptp4a2 H3088F03  −2.118 1.615 
 Rho-associated coiled-coil Rock1 H3069C09  −2.808 3.183 
 TNF receptor associated factor Traf1 H3015E06 NF-κB dependent −2.011 1.243 
Nuclear proteins/transcription factors      
 Cbp/p300 interacting transactivation Cited4 H3076H08 NF-κB co-activator −2.178 2.745 
 High mobility group box 1 Hmgb1 H3126A05 Binds p50 subunit −3.104 1.739 
 Jun oncogene Jun H3058C09 NF-κB co-activator −2.906 2.057 
 Ras-related C3 Rac1 H3018C09 Inducer of NF-κB −2.004 3.329 
RNA processing      
 Nuclear protein 220† Np220 H3029A07  −2.259 1.827 
 RNA polymerase II* Rpo2-3 H3055H08 Coactivator of p65 −2.639 4.501 
Protein synthesis/modification      
 Eukaryotic translation 4g2 Eif4g2 H3113E10  −4.698 2.222 
 Nedd4 WW-binding protein 4* N4wbp4 H3062G06  −6.966 2.814 
Structural proteins      
 Alpha 2 glycoprotein 1*† Azgp1 IMAGE:521249  −2.013 1.398 
 Beta spectrin 2*† Spnb2 H3010G09  −3.781 1.015 
 Catenin beta* Catnb H3031E05 Regulated by IKK −3.998 3.061 
 Fibronectin† Fn1 H3116A10 NF-κB site in promoter −6.201 4.723 
 Catenin α 1*† Catna1 H3018E08  −1.996 1.723 
 Tenascin C Tnc L0062E01 NF-κB site in promoter −2.557 1.956 
Other      
 Deleted in polyposis Dp1 J0420H06  −2.473 2.137 
 Insulin-like growth factor r2 Igfr2 H3148G08  −2.765 2.494 
 Ninjurin 1* Ninj1 H3072B10  −2.579 4.878 
 Rabaptin 5*† Rab5ep H3002C01  −2.739 1.333 
 Topoisomerase II α*† Top2a H3139A05 Inducer of NF-κB −2.427 2.893 
 Tumor differentially expressed† Tde11 H3014H10  −2.593 1.18 
 WW domain binding 5* Wbp5 H3127H02  −2.087 3.313 
 Zinc finger protein 68*† Zfp68 H3058F07  −4.122 5.885 

NOTE. Number of genes regulated by NF-κB = 47/141. Number of genes previously associated with NF-κB = 39/141.

*

Genes containing kB site in promoter region.

†

Genes containing ACTACAG motif in coding sequence.

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©2004 American Association for Cancer Research.
2004