In the article by W. Wang et al., entitled “Single Cell Behavior in Metastatic Primary Mammary Tumors Correlated with Gene Expression Patterns Revealed by Molecular Profiling,” which appeared in the issue of Cancer Research (pp. 6278–6288), Figures 2 and 3 appeared incorrectly. The correct figures and corresponding legends appear below and to the right.

In the article by V. L. J. Whitehall et al., entitled “Morphological and Molecular Heterogeneity within Nonmicrosatellite Instability-High Colorectal Cancer,” which appear in the issue of Cancer Research(pp. 6011–6014), Group C cancers should have been defined as MINT+ indicating that only 1–2 MINT markers were methylated.

Fig. 2.

Carcinoma cells in MTLn3 tumors adhere to ECM, extend pseudopods, and exhibit linear excursions toward blood vessels. Carcinoma cells in MTC (A) and MTLn3 (B–E) tumors were imaged by multiphoton microscopy. A, carcinoma cells in MTC tumors do not attach or orient on collagen-containing ECM fibers in vivo. A single fiber can be seen bending around the cell. The arrow points to collagen fiber bent over cell. B, carcinoma cells in MTLn3 tumors are observed to contact collagen-containing fibers. Arrowheads point to cell-matrix interactions. Elongated cells are highlighted by brackets. Scale bar, 25 μm. C, carcinoma cell is seen attached to matrix fiber (black arrowhead in C). The cell is delineated by the white dotted line. D, 4 min later, the carcinoma cell has spread along the fiber by approximately 8 μm. The red arrowhead shows the position of attachment after 4 min. The black arrowhead in D indicates the original attachment site in C. Scale bar, 10 μm. E, in a stereo pair image, GFP-labeled carcinoma cells are observed entering a rhodamine-dextran-filled blood vessel. The cells (green) are seen on the surface of the blood vessel (red) with pseudopodia (yellow) elongating into the vessel. Arrows point to cells entering blood vessel. The image is a 120-μm stack of 30 optical planes, each taken with a multiphoton microscope and then rendered as a stereo pair. The initial depth of the image is >100 μm. The blood space was labeled by injecting 2 M Dalton rhodamine-dextran i.v. into the blood space. Scale bar, 25 μm.

Fig. 2.

Carcinoma cells in MTLn3 tumors adhere to ECM, extend pseudopods, and exhibit linear excursions toward blood vessels. Carcinoma cells in MTC (A) and MTLn3 (B–E) tumors were imaged by multiphoton microscopy. A, carcinoma cells in MTC tumors do not attach or orient on collagen-containing ECM fibers in vivo. A single fiber can be seen bending around the cell. The arrow points to collagen fiber bent over cell. B, carcinoma cells in MTLn3 tumors are observed to contact collagen-containing fibers. Arrowheads point to cell-matrix interactions. Elongated cells are highlighted by brackets. Scale bar, 25 μm. C, carcinoma cell is seen attached to matrix fiber (black arrowhead in C). The cell is delineated by the white dotted line. D, 4 min later, the carcinoma cell has spread along the fiber by approximately 8 μm. The red arrowhead shows the position of attachment after 4 min. The black arrowhead in D indicates the original attachment site in C. Scale bar, 10 μm. E, in a stereo pair image, GFP-labeled carcinoma cells are observed entering a rhodamine-dextran-filled blood vessel. The cells (green) are seen on the surface of the blood vessel (red) with pseudopodia (yellow) elongating into the vessel. Arrows point to cells entering blood vessel. The image is a 120-μm stack of 30 optical planes, each taken with a multiphoton microscope and then rendered as a stereo pair. The initial depth of the image is >100 μm. The blood space was labeled by injecting 2 M Dalton rhodamine-dextran i.v. into the blood space. Scale bar, 25 μm.

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Fig. 3.

Time-lapse imaging of intravasation of carcinoma cells in MTLn3 tumors. A–F, using multiphoton imaging, a single carcinoma cell (green) is seen crawling along a blood vessel. The arrows show the direction and the distance the cell moved along the vessel. Images were taken 5 min apart. Scale bar, 25 μm. G, the full field is shown to orient the area in which the cell in A–F is shown. Blood vessels are seen as black areas through carcinoma cell cords (green). The white box delineates the area seen in A–F.

Fig. 3.

Time-lapse imaging of intravasation of carcinoma cells in MTLn3 tumors. A–F, using multiphoton imaging, a single carcinoma cell (green) is seen crawling along a blood vessel. The arrows show the direction and the distance the cell moved along the vessel. Images were taken 5 min apart. Scale bar, 25 μm. G, the full field is shown to orient the area in which the cell in A–F is shown. Blood vessels are seen as black areas through carcinoma cell cords (green). The white box delineates the area seen in A–F.

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