Abstract
We have used an assay combining DNA-mediated gene transfer and tumorigenicity in Swiss athymic mice to look for activated ras genes in solid human sporadic melanomas. This assay can detect ras oncogenes mutated at codons 12, 13, or 61. We examined a panel of 13 independent surgical specimens of primary tumors and metastases. No H- or K-ras oncogenes were detected; an N-ras oncogene, mutated at codon 61, was identified in one of the 13 samples. No N-ras genes mutated at codon 13 were detected. Thus, the tumorigenicity assay detects a low frequency of ras gene activation in melanomas.
This research was supported by INSERM and by grants from the Fédération Nationale les Centres de Lutte Contre le Cancer, Association pour la Recherche contre le Cancer, the Fondation pour la Recherche Médicale, and the Comités Départementaux des Bouches du Rhône et des Hautes-Alpes de la Ligue Nationale Français Contre le Cancer.