Transcription of Genes of the Carcinoembryonic Antigen Family in Malignant and Nonmalignant Human Tissues¹

Normal and diseased human tissues were analyzed for the transcription of genes of the carcinoembryonic (CEA) family. Epithelial tissues of colonic origin, whether malignant or normal, all express two closely related mRNA species of 3.0- and 3.5-kilobase mRNA which code for CEA. Only tissues of colonic origin were found to express these CEA-specific transcripts. Colon carcinomas consistently express a 2.6-kilobase mRNA species as well which codes for nonspecific cross-reacting antigen. Nonneoplastic colon mucosas, on the other hand, express lower or nondetectable levels of this transcript. Most breast carcinomas produce only the nonspecific cross-reacting antigen mRNA, whereas leukocytes of chronic myelogeneous leukemia express both nonspecific cross-reacting antigen mRNA and a 2.3-kilobase mRNA corresponding to a yet undefined gene of the CEA family. Thus the multiple CEA-like products reported to be produced by these tissues correspond to only four different mRNA species coding for three different peptides. These data suggest a less complex organization of the CEA family than was previously suspected and point to posttranscriptional modifications, such as variable patterns of glycosylation, as the likely reason for much of the observed complexity in CEA-like glycoproteins.