The treatment of HeLa cells with various concentrations of sodium β-4-methoxybenzoyl-β-bromacrylate (Cytembena) results in inhibition of growth and modification of cell cycle distribution. These phenomena were observed at concentrations between 7.5 × 10−5 and 2.5 × 10−5m. The estimation of DNA content by flow cytometry showed an important shift in the distribution of cycling cells with a relative decrease of G0 + G1 cells and a striking accumulation of G2 + M cells. According to our experimental conditions, the blocking up in G2 + M is irreversible at 7.5 and 5 × 10−5m.


Supported in part by Centre National de la Recherche Scientifique Grant L. A. 206.

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