This study deals with the effects of gastrin on the incidence of gastric tumors in rats induced by N-methyl-N′-nitro-N-nitrosoguanidine. Inbred Basel-Wistar rats received N-methyl-N′-nitro-N-nitrosoguanidine in drinking water (50 µg/ml for 32 weeks) in order to produce gastric carcinoids. A treatment with s.c. injection of pentagastrin (300 µg/kg, once daily for 4 weeks) was started at the beginning of N-methyl-N′-nitro-N-nitrosoguanidine treatment simultaneously, on the 4th, 8th, 16th, and 32nd week after start of N-methyl-N′-nitro-N-nitrosoguanidine treatment, respectively. At autopsy, from the 55th to 60th week after start of the experiment, only in the eighth-week group of gastrin-treated rats was the incidence of gastric carcinoid significantly higher than in the gastrin-untreated group of rats receiving N-methyl-N′-nitro-N-nitrosoguanidine alone. The incidence of adenocarcinoma in the glandular stomach also was high only in the fourth-week group of gastrin-treated rats. However, these effects could not be seen in other gastrin-treated or untreated groups of rats. The data suggest that gastrin treatment in the early stage of rat stomach carcinogenesis by N-methyl-N′-nitro-N-nitrosoguanidine is effective in increasing the development of gastric tumors.
Supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture.