Platelets from patients with acute myelogenous leukemia, both before and after remission induction, were evaluated for their ability to incorporate d-[U-14C]glucose into the four amino acids, glutamine, asparagine, glutamic acid, and aspartic acid. Normal platelets incorporated about 80% of the activity into the amides, glutamine and asparagine, and only 20% into their respective amino acids, glutamic acid and aspartic acid. Platelets from patients with acute myelogenous leukemia in the acute stage showed a reversal of this pattern, which then returned to normal during remission. However, the concentration of amino acids was higher than normal, suggesting that remission platelets behaved like a young cell population. The abnormal pattern of labeling could be interpreted as a defect in the platelet citric acid cycle thereby compromising its energy source.
Abstract presented at the 58th Annual Meeting of the Federation of the American Societies for Experimental Biology, April 1974. This investigation was supported in part by USPHS Grant CA-11816 from the National Cancer Institute, NIH Research Grant HL-12443, and Leukemia Society of America, Incorporated, N. Y.