The distribution of radioactivity from N-acetyl-9-[14C]-2-aminofluorene and p-[14C]-dimethylaminoazobenzene administered i.v. or p.o. to 18-day-pregnant mice was observed by means of autoradiography of longitudinal section of the whole mouse. Radioactive substances derived from these carcinogens passed through the placenta and were distributed in the fetal organs including the kidney and intestine.

Column, thin-layer, and paper chromatography revealed the presence, in whole fetuses as well as in maternal livers, of N-acety-9-[14C]-2-aminofluorene, N-hydroxy-N-acetyl-9-[14C]-2-aminofluorene, ring-hydroxy-N-acetyl-9-[14C]-2-aminofluorene, and p-[14C]-dimethylaminoazobenzene.

These results establish that N-acetyl-9-[14C]-2-aminofluorene and [14C]-p-dimethylaminoazobenzene are transported, metabolized, and excreted in the mouse fetus.

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This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Ministry of Education.

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