The influence of human serum on in vitro67Ga uptake by L1210 leukemic lymphoblasts has been investigated. Both high- and low-molecular-weight serum components inhibit cellular uptake of the isotope. Inhibition by the high-molecular-weight serum fraction correlates closely with the extent of binding of the radionuclide. Although transferrin participates in high-molecular-weight inhibition, it accounts for 10% or less of the inhibitory and binding capacity. Similarly, various low-molecular-weight serum components, including citrate, phosphate, glutamate, and others, contribute to inhibition. This inhibition of 67Ga uptake by serum results from the presence of several, perhaps many, inhibitory components.

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This research was supported by NIH Grant CA-13148.

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