Studies on Repair of Adenovirus 2 by Human Fibroblasts Using Normal, Xeroderma Pigmentosum, and Xeroderma Pigmentosum Heterozygous Strains Free

Cell strains established from fibroblasts of 10 normal persons, 12 persons afflicted with xeroderma pigmentosum (XP), and 4 XP heterozygotes have been used as hosts in studies on the repair of ultraviolet-irradiated human adenovirus 2. The virus appeared most ultraviolet light sensitive when strains belonging to XP complementation Groups A and D were used as hosts, less sensitive when strains belonging to Groups B and C were used, and least sensitive when normal or heterozygous strains were used. One-hit inactivation of adenovirus 2 required fluences of 7 to 15, 25 to 78, and 222 J/sq m, respectively, in each of these three categories of cell strains. One XP strain, judged by other methods to be capable of normal repair, was found to have a 30% repair defect by the adenovirus repair assay.