Bone marrow cells from 15 children with acute lymphocytic leukemia in remission were preserved in liquid nitrogen for periods of up to 16 weeks. Although a substantial reduction in the number of myeloid cells was noted after cryopreservation, the colony-forming cells were still capable of proliferation. Dimethyl sulfoxide was demonstrated to be superior to glycerol as a cryoprotective agent for human marrow cells.


Supported in part by NCI Grant 5P02 CA 10435, the Children's Hematology Research Association of St. Louis, Mo., and American Cancer Society Grant CI-105.

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