Treatment with bovine enterovirus 1 (BEV-1) in microtest plates of mouse cells derived from Sarcoma 180, Ehrlich ascites, A755, methylcholanthrene-induced epithelial carcinoma, and an L-cell line causes highly significant decreases (18 to 98%) in viable cells, whereas cells derived from spleen, lung, thymus, liver, and kidney of normal mice showed no significant change after exposure to virus. Application of the method to cells derived from human tumors revealed decreases of 0 to 51% whereas a variety of normal tissues responded by decreases from 0 to 39%. However over one-half the human tumor preparations responded with decreases greater than 17% whereas only 4 of 22 normal preparations showed declines of that magnitude.

The susceptibility of rabbit and mouse kidney cells to BEV did not change over 3 (mouse) to 12 (rabbit) passages in culture. However the “normal” cells of the continuous lines Vero, BSC-1, and LLC-RK1 were susceptible to the virus in both the microtest plate and plaque assays.

Treatment of normal mice, rabbits, and dogs with BEV-1 by intracranial injection led to no clinical abnormalities, nor could virus be recovered from the brain. Some inconstant changes were noted on pathological examination. Mice bearing Sarcoma 180 and treated by i.p. injection of BEV-1 showed only mild splenic hyperplasia early in the course of treatment.

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This work was supported by grants from the James Whitcomb Riley Memorial Association (Project 71-20), the Little Red Door, the Clinton County Cancer Society, and the Indiana Elks.

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©1973 American Association for Cancer Research.
1973
Cancer Research, Inc.