The activities of seven lysosomal and five nonlysosomal enzymes were measured in several Morris transplantable hepatomas and the livers of the Buffalo and ACI strains of rats, and in rats of the same strains without hepatomas. Significant x2 distributions (p < 0.05) were seen between the lysosomal and nonlysosomal enzyme activities of the hepatoma and the liver in the same animal. A similar positive distribution (p < 0.01) was seen between the lysosomal enzyme activities and the chromosome number. A result approaching significance (p < 0.01) was seen when the hepatoma lysosomal enzyme activity was correlated with both the tumor differentiation and the ability of the tumor to metastasize. The fast-growing hepatomas had a lesser protein content than the slow-growing hepatomas. in general, the average specific activities (units/mg protein) of the lysosomal enzymes (81%) and nonlysosomal enzymes (59%) from hepatomas were greater than those in the livers of the control animals without hepatomas. The livers of the host animals and the hepatomas derived from cells maintained in tissue culture and returned to in vivo growth had the greatest amount of lysosomal enzyme activity.

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Presented in part at the Second Hepatoma Conference, Philadelphia, Pa., June 2–3, 1969, and at the 61st Annual Meeting of the American Association for Cancer Research, Chicago, Ill., April 8 to 10, 1971. This work was supported in part by an American Cancer Society Institutional grant and by Damon Runyon Cancer Fund Grant GR-926.

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