Effects of 5-fluorouracil, Methotrexate, cytosine arabinoside, 6-mercaptopurine, cortisol, and cortisol acetate were studied in six established cell lines derived from the buffy coat of different normal individuals and from patients with various malignant diseases. Each agent, except for cortisol and cortisol acetate, showed characteristic and stable dose response patterns in every cell line tested.

Since the 50% growth inhibitory concentration of 6-mercaptopurine alone was positively correlated with doubling time of experimental culture, differences in cell line sensitivity to this agent could not be detected.

Cell lines did differ significantly from one another in sensitivity to 5-fluorouracil, Methotrexate, and cytosine arabinoside. The different levels of sensitivity of the various cell lines toward a given agent did not lie in a continuous spectrum, but could be grouped into discrete classes. It is suggested that these sensitivity classes may be related to actual genetic differences between the cell lines.

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©1969 American Association for Cancer Research.
1969
Cancer Research, Inc.