The biologic and biophysical properties of intact and ethyl ether-extracted Rauscher leukemia virus were studied. Treatment of concentrated Rauscher mouse plasma virus with ether results in a rapid loss of infectivity, but the aqueous phase contains viral nucleoids which are still infectious. Bioassay of subpassaged progeny virus of mice inoculated intracranially with ether-extracted Rauscher virus revealed essentially the same leukemogenic response as that of intact virus.

Centrifugation experiments on potassium tartrate gradients with 32P-labeled intact and ether-extracted tissue culture virus (JLS-V10) showed a shift in the radioactive peak from the 1.16 density of intact virus to a greater buoyant density of 1.28 with the nucleoids. Electron microscope examination of the high density material revealed only nonenveloped virus particles.

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This work was conducted under contract No. PH 43-66-98 within the special virus leukemia program of the NIH, USPHS, Bethesda, Maryland.

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©1969 American Association for Cancer Research.
1969
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