Investigation into the nature of the nucleolus has been limited to histochemistry, electron microscopy, or to chemical analysis of nucleolar fractions of disrupted cells. In this study, fluorescent antibody technics were employed with human serum antinucleolar factors to characterize the structure of the organelle. Six immunofluorescent patterns were observed. Using selected highly specific sera with antinucleolar activity, three major antigenic structures were delineated in much the same distribution as previously described by light and electron microscopy: the pars amorpha, the nucleolema, and a distinct perinucleolar area.

1

Supported by Maine Arthritis Foundation Grant #2.

2

Presented in part at the Annual Meeting of the American Rheumatism Association, June 1967, New York, New York.

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©1969 American Association for Cancer Research.
1969
Cancer Research, Inc.