Quantitative Tumorigenicity Assays Using the Anterior Chamber of the Mouse Eye¹

Using a quantitative technic for depositing a definite number of carcinoma or sarcoma cells into the anterior chamber of the mouse eye, without leakage as proven by complete retention of Trypan blue dye, this route generally required a 100- to 1,000-fold lower dose of cells to produce tumors in 100% of animals, as compared to the intramuscular, intravenous, and intraperitoneal routes of inoculation in both irradiated and nonirradiated mice. Neoplastic transformation of a cultured mouse cell line was demonstrated using the quantitative anterior chamber technic.