The influences of collagen, l-hydroxyproline, fibrinogen, egg albumin, calf serum, and muramidase on tumor growth were studied in relation to the growth-promoting activity of chondroitin sulfate using solid hypotetraploid Ehrlich ascites tumor.

One ml of the solutions of substances tested, chondroitin sulfate, and the mixtures of the substance and chondroitin sulfate were given, respectively, s.c. into the back of SMA and ddN mice, followed immediately by the injection of Ehrlich tumor. Average tumor weights in experimental groups were compared with those in control groups, which were given isotonic saline on Day 8 after tumor inoculation.

The effect of chondroitin sulfate, which stimulated the growth of tumor, was markedly inhibited by egg albumin, calf serum, and muramidase. It was, however, only weakly inhibited by a collagen preparation, l-hydroxyproline, or fibrinogen, though they tended to inhibit the tumor growth.

It may be considered that inhibitory effect of these substances on the growth-promoting activity of chondroitin sulfate was due to the decrease of the negative electric charge of chondroitin sulfate.

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