Homogenates of human leukocytes reduced dihydrofolate more rapidly than folate. A correlation was observed between the assay of the enzyme with folate and with dihydrofolate. Homogenates of cells from patients receiving methotrexate reduced dihydrofolate much more rapidly than folate. Uninhibited enzyme was observed with both substrates in some homogenates of cells from patients receiving methotrexate. The level of enzyme activity in chronic myelogenous leukemia was correlated with the white blood cell count of the patient. Low levels of enzyme activity were observed in leukocytes from patients with myeloid metaplasia.

1

This investigation was supported by Contract No. PH-43-62-169 from the Cancer Chemotherapy National Service Center of the National Cancer Institute and by Grant No. 6516 from the NIH.

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©1967 American Association for Cancer Research.
1967
Cancer Research, Inc.