The effect of vincristine sulfate (VCR)2 on the incorporation of isotopic precursors into protein, RNA, and DNA of the protozoan Tetrahymena pyriformis has been studied. DNA synthesis in logartithmically growing cells of T. pyriformis was almost completely inhibited by concentrations of VCR which only moderately depressed RNA and protein synthesis. Under appropriate conditions this selective inhibition of DNA synthesis may be prevented by riboflavin or flavin mononucleotide. Flavin adenine dinucleotide was without effect.

1

Supported by USPHS Research Grant No. CA 07295-02.

2

The following abbreviations are used: VCR, vincristine sulfate, TCA, trichloracetic acid; FMN, flavin mononucleotide; VLB, vinblastine.

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©1966 American Association for Cancer Research.
1966
Cancer Research, Inc.