Marked alterations in cell permeability were brought about by the presence of the detergent Tween 80 in preparations of Ehrlich-Lettré ascites cells. The growth of these cells was normal in host mice after treatment and thus they were viable as transplants. The permeability of the cells increased greatly, as shown by the uptake of Lissamine green. Soluble nucleotides and amino acids were removed by the Tween treatment. Oxygen uptake was reduced by 50%. The incorporation of formate into protein and deoxyribonucleic acid (DNA) decreased, but it increased into nuclear ribonucleic acid (RNA). Incorporation of 32P into the phospholipids increased greatly and was found to be localized in phosphatidyl serine. The interpretation of the alterations brought about by Tween 80 are discussed in relation to the special properties of the tumor cells.
Supported by grant No. CA 05172 from the National Cancer Institute, USPHS.