Acid-soluble nucleotides were separated and characterized from various leukocyte populations: normal leukocytes; acute myeloblastic and chronic myelocytic leukemia; acute lymphoblastic and chronic lymphocytic leukemia, and polycythemia rubra vera. The following nucleotides were identified: DPN, TPN, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP, GTP, AMP, ADP, ATP.3 Increased amounts of UDPG and UDPAG were found in acute myeloblastic and chronic myelocytic leukemic cells.

1

Work supported by the Veterans Administration and grant H-1069, USPHS, and partially during the tenure of a Postdoctoral Fellowship from the National Cancer Institute, USPHS.

3

The abbreviations used are as follows: DPN and TPN, di- and tri-phosphopyridine nucleotide; AMP, ADP and ATP, adenosine-5′-mono-, di-, and tri-phosphate; UDPG, uridine diphosphoglucose; UDPAG, uridine diphosphoacetylglucosamine; UMP, UDP, and UTP, uridine-5′, mono-, di-, and tri-phosphate; CMP, CDP, and CTP, cytidine-5′-mono-, di-, and tri-phosphate; GMP, GDP, and GTP, guanosine-5′-mono-, di-, and tri-phosphate; IMP, inosine-5′-monophosphate; TEAB, triethylammonium bicarbonate; CML, chronic myelocytic leukemia; CLL, chronic lymphocytic leukemia; AML acute myeloblastic leukemia; ALL, acute lymphoblastic leukemia; DEAE-cellulose, diethylaminoethyl-cellulose.

This content is only available via PDF.