A study in vitro of an androgen/estrogen-induced, dependent leiomyosarcoma of the ductus deferens/epididymal tail complex of the Syrian hamster revealed that hormone sensitivity (dependency) was not reflected in cell culture. In organotypic culture, however, this tumor failed abruptly and completely unless the two sex hormones were incorporated in the culture medium. These findings were interpreted to suggest strongly that “hormone dependency” is a tissue resident phenomenon divorced from the economy of the “whole animal.”
This investigation was supported by Public Health Service Research Grant No. CA-02791-08 from the National Cancer Institute.