Cells from human cervix, vagina, Fallopian tube, and kidney tissue, fetal or adult, were obtained by tryptic digestion and maintained in vitro by serial massive-inoculum passage for a few passages. Colony-forming capacity of cells of early passages was examined directly and by colony-to-colony passage. Viability of cells so revealed was low and not sustained by selection of viable cells. In colonial populations different colony forms were seen according to cell form, orientation, and susceptibility to tryptic dispersion. One colony form seen commonly with cervical cultures was “fibroblast-like,” but no colonies were analogously “epithelial-like.” Colony formation was affected variably by use of mixed serum supplement (bovine fetal and human adult), individual donor source of human serum, basal medium in relation to source of cells, and cell attachment conditions in relation to cell source. When optimum serum supplement and basal medium were employed, effect of medium pH (or bicarbonate concentration) was seen, but an optimum could not be established within narrow limits. The chief conclusion is that, with appropriate medium, colonial culture can be applied to a study of cells from the non-neoplastic human female genitourinary tract.

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Supported by training grant CRTY-5010(C3) and research grant C-4559 of the National Cancer Institute, U.S. Public Health Service, and research grant E-62 of the American Cancer Society, to Dr. Jerome T. Syverton (deceased) who made this research possible.

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