From a heterologously transplanted embryonal carcinoma of the testis in the cheek pouch of the golden hamster (Deac 3 tumor) 182 μg. of testosterone was isolated. Evidence for this identification was obtained through spectroscopic procedures, mixed chromatograms and carrier dilution experiments. Progesterone was tentatively identified by mixed paper chromatography and ultraviolet spectra.

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These studies have been carried out with the assistance of research grants from the American Cancer Society and from the National Institutes of Health, United States Public Health Service, as well as assistance from Aids for Cancer Research, Boston, Mass.

Presented in part at the IVth International Congress of Biochemistry September 5, 1958, at Vienna, Austria.

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