1. An evaluation of a biological (tissue culture) response by strain HeLa cells to 100 human sera was presented.

  2. This response was evaluated by a phase contrast microscopic counting of the number of VP cells formed in the periphery of HeLa colonies (4–5-mm. diameter) after these colonies had been cultivated for 24 hours (37° C.) in 100 per cent human serum nutrients.

  3. The response was higher in a greater percentage of colonies cultivated in serum from cancer patients and notably more so within a male group.

  4. The high response within the male group was shown to be due to the influence of the adenocarcinoma patients.

  5. A method of refining this type of biological sero-diagnosis was proposed.

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This work was supported by a grant-in-aid from the American Cancer Society and by a grant-in-aid from the American Cancer Society administered by Dr. C. M. Pomerat.

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