1. A technic has been developed which measures the protein binding of 9,10-dimethyl-1,2-benzanthracene-9,10-C14 in a reproducible fashion.

  2. The technic is sensitive enough to detect the influence of dose on the total activity, specific activity, and weight of skin protein.

  3. The specific activity of the protein depends upon the dose per unit area and the weight response of the treated area.

  4. The pattern of protein binding has been discussed in terms of a rapid and slow net loss of radioactivity.

  5. When the dose of carcinogen was large enough, there was an increase of protein.

  6. A dose of 9,10-dimethyl-1,2-benzanthracene as low as 1 µg/3.46 sq cm of mouse skin has been shown to act as an initiator.

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This research was supported by Grant C-2399 and Cancer Control Grant CS-9212, National Cancer Institute, National Institutes of Health, Department of Health Education and Welfare.

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