The administration of ascorbic acid, combined with rutin, increased the number of takes of hepatoma transplants in a resistant group of leaden hybrids from 35 to 80 per cent. The resistance of C57BL mice to this tumor was also decreased by this combination of substances. The administration of ascorbic acid alone or of rutin alone was without effect.
In mice in which hepatoma transplants grew, there was a marked decrease in the ascorbic acid concentration in the spleen, even when additional ascorbic acid was given to the animal. In mice which resisted the growth of the hepatoma, the level of ascorbic acid in the spleen was high.
This work was supported by grants from the American Cancer Society, the Jane Coffin Childs Memorial Fund for Medical Research, and the National Cancer Institute, National Institutes of Health, U.S. Public Health Service.