Prenylated Rab acceptor 1 domain family, member 2 (PRAF2) is a novel 19-kDa protein with four transmembrane-spanning domains that belongs the PRAF protein family that plays a role in vesicle trafficking and cargo transport. PRAF2 is strongly expressed in several cultured neuroblastoma (NB) cells (for example, SK-N-SH, SH-SY5Y, LAN-1). NB is a tumor that derived from the precursor cells of sympathetic nervous system and the most common extracranial solid tumor of childhood. Recently, our lab identified PRAF2 as a candidate prognostic marker of NB. In the present study, we examined the function of PRAF2 in cell cycle progression of human NB cells. In order to analyze the role of PRAF2, we generated a stable RNA interference (RNAi) short hairpin (sh) PRAF2 knockdown NB cell line (SK-N-SH shPRAF2). We observed that PRAF2 downregulation suppressed the cell viability of SK-N-SH cells. Moreover, PRAF2 knockdown also inhibited migration of SK-N-SH cells. Notably, PRAF2 downregulation also elevated the protein levels of cyclin-dependent kinase (Cdk) inhibitor p27Kip1 in these cells, therefore suggesting a role for PRAF2 in the regulation of NB cell cycle regulation and cell proliferation. In summary, our results suggest that reduction of PRAF2 protein level inhibits cell proliferation and migration in SK-N-SH cells that renders PRAF2 a promising target for therapeutic intervention.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1621.