Growth inhibitory effects of naphthoquinones type compounds in human origin cancer cell line

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Tabebuia avellanedae(Bignoniaceae)(TA), which is native in South America from Brazil to northern Argentina, is wellknown in traditionalfolk medicne used for the treatment of various disease during five hundred years. The inner bark of this plant produced in Brazil is distributed in Asia as a herb tea and healthy purpose. Previously, we reported that extract essense of TA(TA ess.) and including naphthoquinones type active compounds, lapachol, beta-lapachone and NFD inhibited TPA-induced in vitro assay for anti-tumor promoters and also suppressed the generation of superoxide and nitric oxide by convenient assay method. In this study, the purpose of other potency of these useful samples was to develop new active effect, cytotoxic function as an effective cancer chemosensitivity test, Effects of lapachol, beta-lapachone and NFD on cancer cells growth was investigated using three cell line, A549, PC-3 and MCF-7 which are human derived organ cell line. Physiological concentration of TA ess. had weak growth inhibitory effect in three cell lines, when cells were treated with 0.05 mM for 72 h. In contrast,lapachol and NFD had significantly inhibited dose-dependentin same cell line after 72 h incubation. Western blot analysis for MAPK signaling pathway showed apparent reduction of p38 and MEK in MCF-7 and A549 cell treated with NFD, but PC-3 observed no changes of these selected protein levels through sequential steps. These results suggested that growth inhibitory effects of cancer cell by natural isolated compounds might be related to the induction of MAPK pathway, and these naturally occurring substances might be a potential candidate for further developing
 chemopreventive agent.