The influence of human serum on in vitro67Ga uptake by L1210 leukemic lymphoblasts has been investigated. Both high- and low-molecular-weight serum components inhibit cellular uptake of the isotope. Inhibition by the high-molecular-weight serum fraction correlates closely with the extent of binding of the radionuclide. Although transferrin participates in high-molecular-weight inhibition, it accounts for 10% or less of the inhibitory and binding capacity. Similarly, various low-molecular-weight serum components, including citrate, phosphate, glutamate, and others, contribute to inhibition. This inhibition of 67Ga uptake by serum results from the presence of several, perhaps many, inhibitory components.


This research was supported by NIH Grant CA-13148.

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