Elimination of Leukemic Cells from Human Bone Marrow Using Monoclonal Antibody and Complement

Bast, Robert C.; Ritz, Jerome; Lipton, Jeffrey M.; Feeney, Maryellen; Sallan, Stephen E.; Nathan, David G.; Schlossman, Stuart F.

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Articles| March 01 1983

Elimination of Leukemic Cells from Human Bone Marrow Using Monoclonal Antibody and Complement^¹

Robert C. Bast, Jr.;

Robert C. Bast, Jr.

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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Jerome Ritz;

Jerome Ritz

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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Jeffrey M. Lipton;

Jeffrey M. Lipton

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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Maryellen Feeney;

Maryellen Feeney

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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Stephen E. Sallan;

Stephen E. Sallan

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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David G. Nathan;

David G. Nathan

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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Stuart F. Schlossman

Divisions of Tumor Immunology [R. C. B., J. R., M. F., S. F. S.], Medicine [R. C. B.], and Pediatric Oncology [J. M. L., S. E. S., D. G. N.], Sidney Farber Cancer Institute

; the

Department of Medicine [R. C. B., J. R., S. F. S.], Brigham and Women's Hospital

; the

Department of Medicine [R. C. B., J. R., S. F. S.] and Pediatrics [J. M. L., S. E. S., D. G. N.], Harvard Medical School

,

Boston, Massachusetts 02115

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Author & Article Information

²

Scholar of the Leukemia Society of America. To whom requests for reprints should be addressed, at Sidney Farber Cancer Institute, 44 Binney Street, Boston, Mass. 02115.

³

Special Fellow of the Leukemia Society of America.

⁴

Dyson Foundation Investigator in Pediatric Oncology.

Received: June 01 1982

Accepted: October 29 1982

Online ISSN: 1538-7445

Print ISSN: 0008-5472

1983

Cancer Research, Inc.

Cancer Res (1983) 43 (3): 1389–1394.

Abstract

Human leukemic cells which bear the common acute lymphoblastic leukemia antigen can be lysed with a murine monoclonal antibody (J-5) in the presence of rabbit complement. Conditions have been defined for eliminating ⁵¹Cr-labeled common acute lymphoblastic leukemia antigen-positive NALM-1 cells or cryopreserved leukemic lymphoblasts from a 100-fold excess of human bone marrow. Optimal lysis is obtained with treatment for a total of 90 min. Three treatments for 30 min are more effective than two treatments for 45 min or one treatment for 90 min. Separation of marrow on a Ficoll:diatrizoate gradient does not permit more effective elimination of leukemic cells. Tumor cell lysis is inhibited by high concentrations of common acute lymphoblastic leukemia antigen-positive cells (2 × 10⁷/ml) and by high concentrations of bone marrow (10⁸/ml). Under optimal conditions, >99% of ⁵¹Cr-labeled leukemic lymphoblasts can be eliminated from a 100-fold excess of human marrow. Selective removal of leukemic cells from human bone marrow in vitro should facilitate trials of autologous marrow transplantation.

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1983

Cancer Research, Inc.

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Elimination of Leukemic Cells from Human Bone Marrow Using Monoclonal Antibody and Complement^¹

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Elimination of Leukemic Cells from Human Bone Marrow Using Monoclonal Antibody and Complement^¹