The segregation of dispersed cellular particles obtained from human lymph nodes has been carried out using a ten-step centrifugation procedure and a fluid medium consisting of 0.1 M phosphate buffer, pH 8.0, containing 0.03 per cent or 0.3 per cent heparin. Nuclear, mitochondrial, and submicroscopic particle fractions are obtained at suitable gravitational forces.

An analysis of the particle populations of the high gravity centrifugation sediments indicates the presence of polydispersed particles ranging from 10 to 280 mµ in neoplastic and non-neoplastic lymph nodes. The predominant particle size obtained from Hodgkin's lymph nodes, 10–20 mµ, differs significantly from the particle sizes found in the non-neoplastic lymph nodes.

The presence of ribose nucleic acid and the absence of desoxyribose nucleic acid was demonstrated in the high gravity sediments. The per cent nucleic acid in high gravity sediments of neoplastic and non-neoplastic lymph nodes were compared.

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©1950 American Association for Cancer Research.
1950
Cancer Research, Inc.
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