Estrogen receptor (ER)-β signaling is associated positively in colon tumor progression, whereas downregulation or loss of function of retinoid X receptor (RXR)-α occurs in colon tumors. The chemopreventive efficacies of the estrogen antagonist raloxifene and the selective RXR agonist bexarotene were tested individually and in combination, during promotion and progression stages of colon tumorigenesis. Colon tumors were induced in male F344 rats with azoxymethane and at early adenoma stage, groups of rats (36 or 45 per group) were fed diets containing raloxifene (1.5 or 3 ppm), bexarotene (50 or 100 ppm), or their low-dose combinations for 40 weeks. Raloxifene or bexarotene alone significantly suppressed colon adenocarcinoma formation in terms of multiplicities (mean ± SE): control, 3.59 ± 0.25; 1.5 ppm raloxifene, 2.51 ± 0.29 (P < 0.004); 3 ppm raloxifene, 2.14 ± 0.28 (P < 0.0001); 50 ppm bexarotene, 2.25 ± 0.32 (P < 0.001); 100 ppm bexarotene, 2.1 ± 0.27 (P < 0.0001); and 1.5 ppm raloxifene + 50 ppm bexarotene, 1.57 ± 0.21 (P < 0.0001). The low-dose combination caused significant (56%) inhibition of adenocarcinomas as compared with control diet fed rats. Tumors exposed to raloxifene, bexarotene and/or the combination showed significant suppression of proliferating cell nuclear antigen, cyclin D1, and β-catenin with an increased apoptotic cells (3-fold) and p21 expression (3.8-fold) as compared tumors of rats fed control diet. The combination of low doses of raloxifene and bexarotene significantly suppressed the progression of colonic adenomas to adenocarcinomas and may be useful for colon cancer prevention and/or treatment in high-risk individuals. Cancer Prev Res; 6(12); 1251–61. ©2013 AACR.

Colorectal cancer is the third most common cancer diagnosed in both men and women in the United States. The American Cancer Society's most recent 2013 estimates are 102,480 new cases of colon cancer and 40,340 new cases of rectal cancer. Overall, the lifetime risk of developing colorectal cancers is about 1 in 20. Although 90% of the cases of the disease occur in people older than 50 years, studies show that colorectal cancer is on the increase in younger patients. Changes in lifestyle, hormone replacement therapies, and environmental hormone mimics may be playing a role in this increase in colorectal cancer in young adults.

The biologic effects of estrogen are mediated by its binding to one of the structurally and functionally distinct estrogen receptor (ER)s (ER-α and -β). ER-β is the predominant ER in the human colonic epithelium (1). There are opposing results with respect to the expression and roles of ERs in colon cancer. Some studies reported that the levels of ER-β are reduced in colorectal cancer compared with normal colonic tissue; and it has been suggested that these decreased levels may be related to loss of differentiation and advanced Dukes staging (2). In contrast, we and others have found that ER-β is associated with positive signaling in animal and human colon carcinomas (3–5). Chlebowski and colleagues (6) observed an increased number of positive lymph nodes and more advanced regional and metastatic disease in women who were on hormone treatment compared versus placebo (76.2% vs. 48.5%; P = 0.004) in a Women's Health Initiative (WHI) trial of estrogen plus progestin in postmenopausal women. Also, more women in the hormone group had metastatic colorectal cancer compared with those taking placebo. Another study reported no statistical difference in colorectal cancer between a hormone therapy group and a placebo group (7). Two other prospective studies reported that high concentrations of circulating estrogen conferred increased risk for colorectal cancer (8, 9). A recent case-controlled study reported that men with both an estrogen receptor 2 gene ESR2 genetic variant (rs4986938 risk genotype) and high estradiol level had a higher risk for developing colorectal cancer (10). A recent report by Simon and colleagues (11) showed that an estrogen plus progestin group had no reduced colorectal cancer mortality; rather, they observed a nonsignificant increase in colorectal cancer deaths compared with placebo (37 vs. 27 deaths;P = 0.320). The selective estrogen receptor modulators (SERM) exhibit specific ER agonistic and antagonistic activity by binding to ER-α and/or ER-β (12). Of the SERMs, raloxifene has antiestrogenic effects on the breast and bone, but it does not have an estrogenic effect on the uterus (12). We have reported inhibition of early precursor lesions in colon by raloxifene (3) and others reported inhibition of carcinogen-induced mammary carcinoma (13) in animal models.

Retinoid X receptors (RXR) and retinoic acid receptors (RAR) are nuclear receptors that mediate the biologic effects of retinoids by their involvement in retinoic acid–mediated gene activation. We and others have identified downregulation or loss of function of RXR-α in preclinical and clinical colon tumors (14). We previously have reported increased expression of RXR-α and inhibition of colon tumors in ApcMin/+ mice treated with the selective RXR agonist bexarotene and increased RXR-α (15), and inhibition of azoxymethane-induced colonic ACF formation in F344 rats treated with β-ionone, present in vegetables and fruits (14). Thus, increased RXR-α expression and decreased ER-β expression might favor colon tumor growth. RXR receptor is reported to repress estrogen-responsive genes activated by ER in human breast cancer cells indicating a cross-talk between ER and RXR receptors functions (16). RXRs form heterodimers with various nuclear receptors and bind to their response elements and have the potential to interact with signaling pathways either negatively or positively. Thus, synergy between ER and RXR is not confined to single pathway mechanism. As reported by Suh and colleagues (17), bexarotene suppress ER-positive breast cancers by synergizing with SERMs through TGF-β pathway in rat models. In a colorectal cancer, multiple genes involving various signaling pathways are deregulated. It is noteworthy that colon tumors overexpress ER-β and frequently associated with loss of expression of RXR-α. Hence, developing ER-β antagonist and RXR-α agonist combination is considered to be a rational approach for colon cancer prevention.

U.S. Food and Drug Administration (FDA) had approved raloxifene for treatment of osteoporosis and invasive breast cancer; whereas bexarotene for cutaneous T-cell lymphoma. Bexarotene also has been used off-label for lung cancer, breast cancer, and Kaposi's sarcoma. As discussed above, molecular pathogenesis of colon tumor progression suggests a progressive loss of RXR-α and an increase in ER-β expression. We hypothesize that upregulation of RXR-α in association with downregulation ER-β expression may provide better chemopreventive efficacy. Here, we evaluated the dose response and combinational chemopreventive efficacies of raloxifene and bexarotene during promotion and progression stages of colon adenocarcinoma formation in F344 rats.

Chemicals

Raloxifene and bexarotene were provided by the Division of Cancer Prevention (DCP) Repository at the National Cancer Institute (Rockville, MD). Primary antibodies (monoclonal/polyclonal) to β-catenin, cyclin E, cyclin D1, cdk2, Cdc25c, p21, and proliferating cell nuclear antigen (PCNA) were from Santa Cruz Biotechnology. Horseradish peroxidase–conjugated secondary antibodies were from Santa Cruz Biotechnology. A human tissue array for colorectal cancer was obtained from Cybrdi.

Immunohistochemistry of a human colorectal adenocarcinoma tissue array for expression of RXR-α and ER-β

A colorectal carcinoma tissue array consisting of 72 formalin-fixed cores from 69 different patients was used to examine protein expression patterns for RXR-α and ER-β. Tissue array sections (5 μm) were dried at 56°C, deparaffinized in xylene (15 minutes twice), rehydrated, and washed with PBS for 15 minutes at room temperature. Specimens were treated in a water bath in 0.01 mol/L citrate buffer (pH 6.0) for 30 minutes at 100°C, slowly cooled to room temperature, and washed with PBS for 5 minutes at room temperature. After quenching endogenous peroxide with 3% hydrogen peroxide in PBS for 10 minutes at room temperature, the sections were incubated with a blocking solution (supplied with the Zymed kit) for 60 minutes at room temperature. Then, the slides were incubated overnight at 4°C with a 1:300 dilution of anti-RXR-α or a 1:200 dilution of anti-ER-β (Santa Cruz Biotechnology). After several washes with PBS, the slides were incubated with secondary antibody for RXR-α and ER-β for 2 hours. The color reaction was developed using 3,3′-diaminobenzidine (DAB) according to the manufacturer's instructions given in the kit supplied by Zymed Laboratories. The percentage of immunostained cells was graded as follows: 0 (no staining); 1 (>0%–5%); 2 (>5%–50%); and 3 (>50%).

Animals and experimental diets

Weanling male F344 rats obtained from Harlan Breeding Laboratories were randomly distributed by weight into control and experimental groups. Animals had access to food and water at all times. Food cups were replenished with fresh diet twice weekly. All ingredients for the semipurified diets were purchased from Bioserv and stored at 4°C before diet preparation. Diets were based on the modified American Institute of Nutrition (AIN)-76A diet. Modified AIN76A diet contains corn starch 52%, casein 20%, mineral mix 3.5%, dextrose 13%, vitamin mix 1%, cellulose 5%, dl-methionine 0.3%, choline bitartrate 0.2%, corn oil 5%. Bexarotene and/or raloxifene was premixed with a small quantity of diet and then blended into bulk diet using a Hobart mixer. Both control and experimental diets were prepared weekly and stored in a cold room. Agent content in the experimental diets was determined periodically in multiple samples taken from the top, middle, and bottom portions of individual diet preparations to verify uniform distribution.

Bioassay: chemopreventive efficacy evaluation

Beginning at 5 weeks of age, all rats were fed the modified AIN-76A diet. At 7 weeks of age, the animals were given subcutaneous injections of azoxymethane at a dose of 15 mg/kg body weight or saline as solvent control once weekly for 2 weeks. Nine weeks after the azoxymethane injections, animals were maintained on AIN-76A diet or AIN-76A diet containing 1.5 ppm or 3 ppm of raloxifene, 50 or 100 ppm of bexarotene, or their low-dose combinations for 40 weeks. The dose of raloxifene was based on our previous 8-week study using ACF as an endpoint, and the dose of bexarotene was based on our previous study with ApcMin mice (3, 17). At termination, animals were killed by CO2 asphyxiation and the colons were removed, rinsed in PBS, opened longitudinally, and flattened on a filter paper. The location and size of each tumor were noted. Mucosal scrapings were collected and stored at −80°C for further analysis. Tumors were removed, snap frozen, or fixed in 10% buffered formalin for 24 hours and transferred to 80% ethanol for histopathologic analysis.

Histopathology and immunohistochemistry

The tumor tissues were fixed in 10% formalin, dehydrated, embedded in paraffin, and cut into 4-μm-thick sections. For histopathology, the sections were hydrated and stained with hematoxylin and eosin according to the standard protocol. The stained sections were analyzed for tumor grade by a pathologist blinded to the treatments. Protein markers were evaluated in control and treated sections by immunohistochemical analysis as reported previously. Sections were incubated with primary antibodies against PCNA (1:1,500), cyclin D1 (1:500), β-catenin (1:500), p21 (1:500), ER-β (1:500), and RXR-α (1:500) and then with appropriate secondary antibodies. Random images were taken with a bright-field microscope (Olympus AX71) connected to a digital imaging system with SPOT RT software version 3.0. Scoring of PCNA-positive cells in the tumors was performed by two investigators blinded to the identity of the samples (light microscopy at 400× magnification). Cells with a brown nucleus were considered positive. The proliferation index was determined by dividing the number of positive cells by the total cells and multiplying by 100.

Apoptosis assay

Sections of 5 μm thickness were cut, mounted on slides, rehydrated, and stained using the terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL) method as previously described. TUNEL-positive cells were visualized by chromogenic staining with DAB and slides were counterstained with methyl green. Stained apoptotic epithelial cells (a minimum of 10 microscopic fields per section) were counted manually in a single-blind fashion.

Protein expression by Western blot analysis

Tissues exposed (24 hours) to various concentrations of raloxifene, bexarotene and combinations were harvested by gentle scraping and processed for protein and estimated protein content using the Bio-Rad Protein Assay reagent as mentioned previously. An aliquot (50 μg protein/lane) of the total protein was subjected to SDS-PAGE (10% or 15%), and proteins were transferred to nitrocellulose membranes. After blocking in 5% milk, the membranes were incubated with primary antibodies to β-catenin, cyclin E, cdk2, Cdc25c, p21, and PCNA (1:500, in TBS-Tween 20 solution), then probed with horseradish peroxidase–conjugated secondary antibody. Detection was done using the SuperSignalWest Pico Chemiluminescence procedure developed by Pierce. The bands captured on Ewen Parker Blue sensitive X-ray films were analyzed by densitometry using image quant software. Immunoblotting with tubulin antibody was done to confirm equal protein loading.

ER-β, β-catenin, cyclin D1, IL-4, and IL-6 messenger RNA expression by reverse transcription PCR

Total RNA from tumor samples was extracted using TRIzol (Ambion) as per the manufacturer's instructions. Equal quantities of DNA-free RNA were used in reverse transcription reactions to make complementary DNA using SuperScript reverse transcriptase (Invitrogen). PCR was carried out for ER-β, β-catenin, cyclin D1, interleukin (IL)-4, and IL-6 using the following conditions: ER-β primers and conditions were as used in the report by Price and colleagues (18). β-Catenin and IL-4 denaturation was carried out at 94°C for 3 minutes, followed by 35 cycles at 94°C for 30 seconds, 55°C for 1 minute, and 72°C for 1 minute. Oligonucleotide primer sequences used for β-catenin were as follows: 5′-CGGGATCCACAAGAAACGGCTTTCA-3′ (sense) and 5′-GAGAATTCCAGGTCAGTATCAAACCA-3′ (antisense). IL-4 oligonucleotide primer sequences used were as follows: 5′-GCCCCCACCTTGCTGTCACC-3′ (sense) and 5′-GCGAAGCACCCTGGAAGCCC-3′ (antisense) and IL-6 oligonucleotide primer sequences used were as follows: 5′-GACTGATGTTGTTGACAGCCACTGC-3′ (sense) and 5′-TAGCCACTCCTTCTCTGTGACTCTAACT-3′ (antisense). IL-6 denaturation was done at 94°C for 30 seconds, followed by 35 cycles at 94°C for 30 seconds, 56°C for 1 minute, and 72°C for 1 minute. Cyclin D1 denaturation was done at 94°C for 3 minutes, followed by 35 cycles at 94°C for 30 seconds, 60°C for 20 seconds, and 72°C for 45 seconds. The oligonucleotide primer sequences used for the cyclin D1 gene were as follows: 5′-CTGGCCATGAACTACCTGGA-3′ (sense) and 5′-GTCACACTTGATCACTCTGG-3′ (antisense). PCR was done using the Taq polymerase Master Mix (Qiagen). The PCR products were visualized and photographed under UV illumination.

Statistical analysis

Tumor multiplicity, defined as mean number of tumors per rat, was analyzed by unpaired Student “t” test with Welch correction. The tumor incidence (percentage of rats with colon tumors) was analyzed by Fisher exact two-tailed test. The data are presented as means ± SEM. Differences between body weights were analyzed by ANOVA. Differences between groups are considered significant at P < 0.05. All statistical analysis was conducted using GraphPad Prism Software 5.1 (GraphPad Software, Inc.).

Expression of RXR-α and ER-β in human colorectal tissue

As a first step toward evaluating the role of RXR-α and ER-β in colorectal cancer, we examined expression of the two receptors in a tissue array consisting of samples from patients (male and female from 25 to 79 years of age) diagnosed with adenocarcinoma, grades 1 to 3. Strong positive staining was seen with an RXR-α antibody in normal mucosal tissue but very weak or no staining was observed in tumors, irrespective of sex or age (Fig. 1A, top). ER-β showed strong/granular staining in tumor tissue with very weak to no staining in normal tissue (Fig. 1A, bottom). The quantitative analysis of ER-β antibody staining showed a majority of patients (94%) colon adenocarcinomas had overexpression (41% score of 3, 35% score of 2, and 17% scored of 1) as compared with patients' normal appearing colonic mucosa showing without any expression (Table 1). In contrast, quantitative analysis of RXR-α antibody staining showed in all the normally appearing colonic tissue samples; as compared with no expression in 65% of colon adenocarcinomas and a modest (score 1) expression in remaining patient's colonic tumors (Table 1). These findings clearly suggest that colon tumor progression was associated with overexpression of ER-β and downregulation of RXR-α expression.

Figure 1.

A, immunohistochemical analysis of ER-β and RXR-α expression in normal human colon crypt and adenocarcinoma tissues. Chemopreventive effects of raloxifene, bexarotene, or their low-dose combination on azoxymethane (AOM)-induced colon tumors in F344 rats. B, experimental design for the bioassay. C, body weights of animals on experimental versus control diets. D, adenocarcinoma multiplicity (number of tumors per rat). E, incidence (% rats with colon tumors).

Figure 1.

A, immunohistochemical analysis of ER-β and RXR-α expression in normal human colon crypt and adenocarcinoma tissues. Chemopreventive effects of raloxifene, bexarotene, or their low-dose combination on azoxymethane (AOM)-induced colon tumors in F344 rats. B, experimental design for the bioassay. C, body weights of animals on experimental versus control diets. D, adenocarcinoma multiplicity (number of tumors per rat). E, incidence (% rats with colon tumors).

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Table 1.

Expression of ER-β and RXR-α in human colorectal adenocarcinomas by immunohistochemical analysis

ER-β expressionRXR-α expression
Percentage stainingScoreNormal-appearing colonic tissuesColon adenocarcinomasNormal-appearing colonic tissuesColon adenocarcinomas
0% 10/12 (83.3%) 1/17 (5.9%) 0/12 (0%) 11/17 (64.7%) 
>0%–5% 2/12 (16.7%) 3/17 (17.6%) 1/12 (8.3%) 4/17 (23.5%) 
>5%–50% 0/12 (0%) 6/17 (35.3%) 3/12 (25%) 2/17 (11.7%) 
>50% 0/12 (0%) 7/17 (41.2%) 8/12 (66.6%) 0/17 (0%) 
ER-β expressionRXR-α expression
Percentage stainingScoreNormal-appearing colonic tissuesColon adenocarcinomasNormal-appearing colonic tissuesColon adenocarcinomas
0% 10/12 (83.3%) 1/17 (5.9%) 0/12 (0%) 11/17 (64.7%) 
>0%–5% 2/12 (16.7%) 3/17 (17.6%) 1/12 (8.3%) 4/17 (23.5%) 
>5%–50% 0/12 (0%) 6/17 (35.3%) 3/12 (25%) 2/17 (11.7%) 
>50% 0/12 (0%) 7/17 (41.2%) 8/12 (66.6%) 0/17 (0%) 

Lack of overt toxicity of bexarotene and raloxifene doses

The maximum-tolerated doses (MTD) for raloxifene and bexarotene were determined previously in F344 rats and ApcMin/+ mice fed the drugs in an AIN-76A diet (3, 17). We used about 20% MTD as the lower doses of raloxifene and bexarotene in the current efficacy study as described (Fig. 1B). Body weights of animals fed the experimental diets containing raloxifene or bexarotene individually or in combination were comparable with those of animals fed the control diet throughout the experimental study, suggesting that the doses of raloxifene, bexarotene or their low-dose combinations did not cause overt toxicity (Fig. 1C).

Raloxifene, bexarotene and their low-dose combinations reduce colorectal cancer tumor incidence and multiplicity

The effects of dietary administration of raloxifene, bexarotene and their low-dose combinations on azoxymethane-induced colon tumorigenesis were evaluated. None of the rats in the saline group (without azoxymethane injection, n = 6) developed tumors when autopsied at week 57 weeks of age (data not shown); however, 97.8% of rats administered with azoxymethane developed colorectal tumors. Raloxifene treatment, at both doses, significantly decreased colon tumor multiplicity: at 1.5 ppm, to 3.28 ± 0.31 (29%, P < 0.004); at 3 ppm, to 2.96 ± 0.30 (36%, P < 0.0006; Fig. 1D). A significant reduction in tumor multiplicity was observed with both low dose (33%, 3.14 ± 0.35, P < 0.003) and high dose (37%, 2.90 ± 0.28, P < 0.0002) bexarotene compared with control diet group. Importantly, low-dose combinations of raloxifene and bexarotene caused 47% inhibition ((2.46 ± 0.27, P < 0.0001) of total colon tumors as compared with control diet (Fig. 1D and E). Raloxifene had a modest effect on the incidence of colon tumors compared with control diet fed rats by 5.6% to 11.2%; but this reduction did not reach statistical significance (Fig. 1E). Similarly, bexarotene at 50 and 100 ppm did not cause significant reduction in colon tumor incidence (8.4%–11.2% decrease as compared with control diet group; Fig. 1E). Thus, neither the low nor high doses of raloxifene or bexarotene caused significant reduction in the colon tumor incidence; but the low-dose combination of these 2 agents caused significant reduction (P < 0.04) in the colon tumor incidence compared with control diet group (Fig. 1E). Colon tumor multiplicity in rats fed control diet was 4.62 ± 0.38 (mean ± SE).

Histopathologic analysis by hematoxylin and eosin staining revealed that 78% of the colon tumors from the control group were adenocarcinomas, and the remaining 22% was adenomas (Fig. 2A–C). Low- and high-dose raloxifene reduced adenomas and noninvasive adenocarcinomas by up to 23%, although this decrease was statistically insignificant as compared with adenomas and noninvasive adenocarcinomas from animals fed control diet. Both doses of raloxifene (1.5 and 3 ppm) reduced the number of invasive adenocarcinomas by about 35% to 53.5% (P < 0.003 to 0.0001; Fig. 2A–C). Like raloxifene, bexarotene insignificantly reduced adenomas (to 0.89 ± 0.15 at 50 ppm; to 0.80 ± 0.13 at 100 ppm) compared with control diet (1.02 ± 0.12). However, bexarotene at both the dose levels caused significant reduction in noninvasive adenocarcinomas (to 0.81 ± 0.12 at 50 ppm, P < 0.0024; to 0.75 ± 0.14 at 100 ppm, P < 0.002) compared with control diet (1.44 ± 0.13). Also, both doses of bexarotene significantly inhibited invasive adenocarcinomas (to 1.44 ± 0.21, P < 0.007 and 1.35 ± 0.19, P < 0.003) compared with control (2.15 ± 0.17). A similar reduction in noninvasive adenocarcinomas was observed with low-dose combinations of bexarotene and raloxifene; the reduction was equal to that achieved with high-dose bexarotene. A greater inhibitory effect (61.8%, P < 0.0001) was observed on invasive adenocarcinomas with these low-dose combinations administered during the promotion and progression stages, as compared with high doses of the individual agents alone (Fig. 2B and C).

Figure 2.

Chemopreventive effects of raloxifene, bexarotene, or combination of low-dose raloxifene plus bexarotene on (A) azoxymethane (AOM)-induced colon adenomas, (B) noninvasive adenocarcinomas, and (C) invasive adenocarcinomas in male F344 rats.

Figure 2.

Chemopreventive effects of raloxifene, bexarotene, or combination of low-dose raloxifene plus bexarotene on (A) azoxymethane (AOM)-induced colon adenomas, (B) noninvasive adenocarcinomas, and (C) invasive adenocarcinomas in male F344 rats.

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Raloxifene, bexarotene and their low-dose combinations decrease cell proliferation and induce apoptosis in colon adenocarcinoma

Because the histologic evaluation revealed that the majority of tumors were adenocarcinomas, the expression of PCNA, a marker for cell proliferation, was determined in the adenocarcinomas from the control and treated groups. The colon tumors from groups fed raloxifene, bexarotene and the combination showed significant reduction of PCNA staining compared with the control group (Fig. 3A). Qualitative microscopic examination of PCNA-stained sections showed a substantial decrease in PCNA-positive cells in tumors from rats exposed to low-dose combinations compared with tumors from rats fed the control diet. Low-dose combinations significantly suppressed proliferation in the colonic tumors as compared with control (Fig. 3A). The quantification of PCNA staining showed 55.1 ± 5.4 (mean ± SEM) PCNA-positive cells in control tumors, as compared with 35 ± 1.4, 30.2 ± 3.4, 19.2 ± 2.4 (means ± SEM) PCNA-positive cells in tumors from raloxifene-, bexarotene- and their low-dose combination–treated mice, respectively, accounting for a decrease in the proliferation index of about 65% (P < 0.0001) with the combination treatment (Fig. 3B). Figure 3C and D summarize the effects of raloxifene, bexarotene and their combinations on tumor cell apoptosis. Qualitative microscopic examination of TUNEL-stained cells showed a substantial increase in TUNEL-positive cells in the tumors of treated rats (Fig. 3C). The quantification of TUNEL-positive cells in polyps from control diet–fed mice showed 6 ± 1. 40 (mean ± SEM), as compared with 9.6 ± 4.38, 14.4 ± 6.24, and 29 ± 5.24 (means ± SEM) positive cells in raloxifene, bexarotene and combination-treated rats, respectively, accounting for an increase in the apoptotic index by >20% (P < 0.0039; Fig. 3D).

Figure 3.

Immunohistochemical analysis of proliferation and apoptotic markers and of ER-β and RXR-α in azoxymethane (AOM)-induced colon tumors after treatment of F344 rats with raloxifene, bexarotene or the combination. A, immunohistochemical staining for PCNA in colon tumors from rats fed control diet or treated with raloxifene, bexarotene or the combination of raloxifene and bexarotene. B, quantification of proliferation in response to the treatments in A. A significant difference was observed in proliferative index between combination-treated and control group tumors. C, TUNEL assay for apoptotic cells in colon tumors from F344 rats fed control diet or treated with raloxifene, bexarotene or the combination of raloxifene and bexarotene. D, quantification of apoptotic assay in D. E, immunohistochemical staining for p21 showing increased expression of p21 in colon tumors from animals given the combination treatment compared with colon tumors from rats fed the control diet. F, immunohistochemical staining for β-catenin showing decreased expression of β-catenin in colon tumors from rats given the combination treatment compared with those from rats fed control diet. G, immunohistochemical staining for cyclin D1 showing decreased expression in colon tumors from rats give the combination treatment compared with those fed control diet. H, immunohistochemical staining for ER-β showing decreased expression in colon tumors from rats given raloxifene, bexarotene or the combination compared with those from rats fed control diet. I, immunohistochemical staining for RXR-α showing increased expression of RXR-α in colon tumors from rats given bexarotene and the combination treatment compared those fed control diet.

Figure 3.

Immunohistochemical analysis of proliferation and apoptotic markers and of ER-β and RXR-α in azoxymethane (AOM)-induced colon tumors after treatment of F344 rats with raloxifene, bexarotene or the combination. A, immunohistochemical staining for PCNA in colon tumors from rats fed control diet or treated with raloxifene, bexarotene or the combination of raloxifene and bexarotene. B, quantification of proliferation in response to the treatments in A. A significant difference was observed in proliferative index between combination-treated and control group tumors. C, TUNEL assay for apoptotic cells in colon tumors from F344 rats fed control diet or treated with raloxifene, bexarotene or the combination of raloxifene and bexarotene. D, quantification of apoptotic assay in D. E, immunohistochemical staining for p21 showing increased expression of p21 in colon tumors from animals given the combination treatment compared with colon tumors from rats fed the control diet. F, immunohistochemical staining for β-catenin showing decreased expression of β-catenin in colon tumors from rats given the combination treatment compared with those from rats fed control diet. G, immunohistochemical staining for cyclin D1 showing decreased expression in colon tumors from rats give the combination treatment compared with those fed control diet. H, immunohistochemical staining for ER-β showing decreased expression in colon tumors from rats given raloxifene, bexarotene or the combination compared with those from rats fed control diet. I, immunohistochemical staining for RXR-α showing increased expression of RXR-α in colon tumors from rats given bexarotene and the combination treatment compared those fed control diet.

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Raloxifene, bexarotene and low-dose combinations downregulate expression of β-catenin, cyclin E, cyclin D1, and cdk2 and increase p21 in colon adenocarcinoma

Raloxifene, bexarotene and low-dose combinations of these agents caused significant inhibition of β-catenin protein expression as evident from both immunohistochemistry and Western blotting results (Fig. 3E). Cyclin D1 also was markedly inhibited by the treatment with low-dose combinations (Fig. 3F). Increased expression of p21 and RXR-α was observed in bexarotene- and combination-treated tumors (Fig. 3G and H). Positive staining for ER-β was observed in both the nucleus and cytoplasm of the control group (Fig. 3I). Surprisingly, no ER-β staining was observed in the bexarotene-treated colon tumors; insignificant staining was observed with raloxifene; and no staining was observed in tumors after the low-dose combination treatment (Fig. 3I). The expression of ER-β mRNA (Fig. 4) correlates with the protein expression results in treated colon tumors. Low-dose combinations also caused a decrease in cdk2 protein expression and cyclin E downregulation in tumors treated with the low-dose combination (Fig. 4). Colon tumors from the control group showed stronger staining for cyclin D1 protein and more cyclin D1 mRNA than did those from the treatment groups, with a strikingly a decreased cyclin D1 difference in low-dose combination group (Figs. 3 and 4). A similar result was seen with ER-β mRNA expression and protein expression in the control tumors (Fig. 5).

Figure 4.

Modulatory effects of raloxifene, bexarotene and their low-dose combinations on β-catenin signaling and cytokine production in azoxymethane (AOM)-induced rat colon tumors. A, Modulatory effects of raloxifene, bexarotene and combinations on β-catenin, cyclin D1, cyclin E, cdk2, and p21 protein expression in colon tumors. The label represents C, duplicates of control colon tumors; R, duplicates of low-dose raloxifene-treated colon tumors; B, duplicates of low-dose bexarotene-treated tumors; R+B, duplicates of low-dose combinations. B–F, Graphs represent the relative protein expression levels of β-catenin, cyclin D1, cyclin E, cdk2, and p21 in treated colon tumors compared with control untreated colon tumors. Significant suppression of ER-β, β-catenin, cyclin D1, cyclin E, and cdk2 and increased expression of p21 was observed in colon tumors treated with the combination. Results shown are means SEM, unpaired “t” test with Welch correction.

Figure 4.

Modulatory effects of raloxifene, bexarotene and their low-dose combinations on β-catenin signaling and cytokine production in azoxymethane (AOM)-induced rat colon tumors. A, Modulatory effects of raloxifene, bexarotene and combinations on β-catenin, cyclin D1, cyclin E, cdk2, and p21 protein expression in colon tumors. The label represents C, duplicates of control colon tumors; R, duplicates of low-dose raloxifene-treated colon tumors; B, duplicates of low-dose bexarotene-treated tumors; R+B, duplicates of low-dose combinations. B–F, Graphs represent the relative protein expression levels of β-catenin, cyclin D1, cyclin E, cdk2, and p21 in treated colon tumors compared with control untreated colon tumors. Significant suppression of ER-β, β-catenin, cyclin D1, cyclin E, and cdk2 and increased expression of p21 was observed in colon tumors treated with the combination. Results shown are means SEM, unpaired “t” test with Welch correction.

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Figure 5.

A, Modulatory effects of raloxifene, bexarotene and combinations on β-catenin, cyclin D1, ER-β, IL-6, and IL-4 mRNA expression in colon tumors in F344 rats. The label represents C, triplicates of control colon tumors; R, triplicates of low-dose raloxifene-treated colon tumors; B, triplicates of low-dose bexarotene-treated tumors; R+B, duplicates of low-dose combinations. B–F, Graphs represent the relative mRNA expression levels of IL-4, β-catenin, cyclin D1, IL-6, ER-β in treated colon tumors compared with control untreated colon tumors. A significant suppression of β-catenin, cyclin D1, ER-β, and IL-6 mRNA was observed in tumors from treated F344 rat. An increased expression of IL-4 was observed in colon tumors with combination treatments. Results shown are means SEM, unpaired “t” test with Welch correction.

Figure 5.

A, Modulatory effects of raloxifene, bexarotene and combinations on β-catenin, cyclin D1, ER-β, IL-6, and IL-4 mRNA expression in colon tumors in F344 rats. The label represents C, triplicates of control colon tumors; R, triplicates of low-dose raloxifene-treated colon tumors; B, triplicates of low-dose bexarotene-treated tumors; R+B, duplicates of low-dose combinations. B–F, Graphs represent the relative mRNA expression levels of IL-4, β-catenin, cyclin D1, IL-6, ER-β in treated colon tumors compared with control untreated colon tumors. A significant suppression of β-catenin, cyclin D1, ER-β, and IL-6 mRNA was observed in tumors from treated F344 rat. An increased expression of IL-4 was observed in colon tumors with combination treatments. Results shown are means SEM, unpaired “t” test with Welch correction.

Close modal

We also tested the effects of these agents on cytokines IL-6 and -4. Although the individual agents did not have much effect on IL-4, combination treatments caused increased IL-4 mRNA expression (Fig. 5). Treatment with bexarotene and with the low-dose bexarotene and raloxifene combination was effective in reducing mRNA for the inflammatory cytokine IL-6 in colon tumors (Fig. 5).

We previously demonstrated that raloxifene and bexarotene prevented azoxymethane-induced ACF in rats and intestinal tumors in APCmin mice (3, 17). Our previous studies showed high expression of ER-β and decreased expression of RXR-α in azoxymethane-induced rat colon tumors. A similar observation was noted in human colon tumor samples (Fig. 1A; refs. 3, 16). Various studies have shown expression of ER in colon cancer cell lines and human colon tissue (5, 19). However, other studies report decreased or no expression of ER in human colon cancer tissue (1, 20, 21), leaving the role of ER in colon cancer complex. Large randomized placebo-controlled trials have suggested that a combination of estrogen and medroxyprogesterone acetate (MPA) resulted in a 37% reduction in the number of colon cancers compared with placebo at 5 years of follow-up (6, 22). However, analysis of this trial also suggested that women who received hormone replacement therapy had more lymph node involvement with aggressive metastatic tumors as compared with those who received placebo (6). However, administration of estrogen alone did not affect significantly the risk of colon cancer development (22, 23). Although few reports suggest protective effect of ER-β in animal models (24–26). In an in vivo study in mice lacking ER-β, administration of azoxymethane or dextran sulfate sodium (DSS) resulted in an increase in colonic tumors (24). In contrast, a recent report by Heijmans and colleagues (27) suggested that estrogen treatment resulted in invasive adenocarcinomas and that the tumor-promoting effects of estrogen were through ER-α and -β. A similar result of increase in tumors with estrogen treatment was observed even in male mice (27). On the basis of the previous reports, raloxifene has been shown to inhibit colon tumor growth in both male and female mice, depending on the endogenous levels of estrogen (28). Although this agent was effective in both the genders, efficacy was more with colon tumors of female mice than with male mice (28). These results suggest a positive role of estrogen in inducing tumor growth and development.

The inhibition of ER-β expression with raloxifene administration during promotion and progression stages of colon tumorigenesis may help to inhibit colon tumor formation, specifically to decrease invasive adenocarcinomas (Fig. 2). These observations suggest that raloxifene halted the tumors at the adenoma and noninvasive adenocarcinoma stages and restricted their progression to invasive adenocarcinomas. These results are similar to observation reported in a STAR trial with raloxifene where invasive breast cancer was inhibited with its use (29). Martinez and colleagues reported a 2-fold increased risk for advanced neoplasia in patients who were classified as high risk previously (30). Therefore, the dosing paradigm used in this study can be used for an intervention trial for these high-risk patients to avoid the spread of disease. It is known that estrogen and ER play a role in proliferation of enterocytes and reports suggest that estrogen and ER increase proliferation of epithelial cells (3, 27). Several clinical trials (STAR; ref. 29), MORE (31), and CORE (32) show that raloxifene does not reduce the risk of ER-negative invasive breast cancer. This result suggests that the raloxifene inhibition of invasive cancers is via the modulation of ER signaling and is consistent with our data showing that control colon tumors have increased ER-β expression and that raloxifene is effective in inhibiting these tumors. We observed decreased expression of PCNA in raloxifene-treated tumors, consistent with inhibition of proliferation. Data analysis from the MORE trial suggests that the risk of colorectal cancer may be reduced for those postmenopausal women taking raloxifene (33).

Bexarotene treatment also resulted in inhibition of ER-β expression (Figs. 3 and 4). A similar observation was reported in a mouse model of lung cancer in which bexarotene inhibited progression (34). Bexarotene is the first synthetic nuclear RXR-selective retinoid approved by the FDA for the treatment of refractory cutaneous T-cell lymphoma in all stages; and a biomarker trial with bexarotene in women with high breast cancer risk is recently completed and no data are yet available of this trial (35). We have reported preventive effects of bexarotene against intestinal tumors in APCMin mice (17) and also have been shown to be profoundly effective as both a preventive and a therapeutic agent in a chemically induced mammary cancer model (36). Previous studies had shown that bexarotene was highly effective in preventing these ER+ tumors (37); and it was effective against ER mammary cancers as well (36, 38). In addition, bexarotene has been shown to have strong synergistic effects with SERMs (39). Thus, suggesting a significant cross-talk between the ER-β and RXR-α. Because raloxifene treatment leads to a decrease in all ER-β isoforms and bexarotene increases RXR-α. A low-dose combination of raloxifene and bexarotene to inhibit colonic tumors based on the mechanistic cross-talk that might provide the synergistic effects. It is noteworthy that low-dose combination of raloxifene and bexarotene provided about 62% inhibition of invasive adenocarcinomas as compared with about 32% inhibition by individual agents; supporting possible cross-talk between ER-β and RXR-α. Bexarotene did not display a striking dose response in inhibiting colon tumor incidence or tumor multiplicity compared with control, although a trend for dose-associated decrease in colon adenocarcinoma was observed. However, at high dose, bexarotene caused significant suppression of both noninvasive and invasive adenocarcinomas compared with control. Low-dose bexarotene suppressed only invasive adenocarcinomas (Fig. 2). This may be due to the antiproliferative effects of bexarotene on the epithelial cells inhibiting invasive capacity of the malignant cells.

The promotion and progression phases of carcinogenesis are associated with increase in cell proliferation and decrease in apoptosis (40, 41). Here, we observed a decrease in cell proliferation and an increase in apoptosis upon treatment with raloxifene, bexarotene and combinations during the promotion and progression stages (Fig. 3). Because stabilization of β-catenin can lead to colon tumor growth (42), we examined the effect of raloxifene and bexarotene on expression of β-catenin and the downstream signaling molecules cyclin D1 and cyclin E-cdk2. β-Catenin and cyclin D1 were downregulated in response to the individual agents and their combination (Figs. 3–5). Brown and colleagues (43) reported that rexinoids suppress premalignant mammary epithelial cell proliferation by G1 cell-cycle block. The combination treatment also caused decreased expression of cyclin E–cdk2, suggesting cell-cycle block in the G1-phase, with increase in p21 (Figs. 3–5).

Colorectal tumors are known for overexpression of inflammatory markers. Previous studies have shown a decrease in inflammatory cytokines in response to raloxifene treatment in healthy postmenopausal women (44). One clinical trial suggested decreased expression of IL-4 with raloxifene use in postmenopausal women. However, differential effects of bexarotene were observed on IL-4 levels in patients versus healthy patients. IL-4 was increased in healthy patients, whereas in patients with a high tumor burden of T-cell lymphoma, a 50% decrease was observed (45). Here, we examined the effects of long-term feeding of raloxifene and bexarotene on inflammatory markers in azoxymethane-induced rat colorectal tumors. Our results are in accordance with the earlier reports of decreased IL-4 in tumors (Fig. 5). Similar to results observed in patients, more IL-4 was observed under conditions of decreased tumor burden in animals given the combination treatment compared with untreated animals. However, we previously have reported decreased expression of IL-4 and -6 in bexarotene-treated colon tumor samples from ApcMin/+ Mice (17). Recently, raloxifene has been reported to have effects on circulating cytokines such as IL-6; but in other studies, effects of raloxifene on these cytokines have been inconsistent (46–49). Our results indicate that bexarotene is more effective than raloxifene in reducing IL-6 in colorectal tumors and that combination of these 2 agents showed a similar effect on IL-6 (Fig. 5).

Overall, our results show that raloxifene inhibited invasive adenocarcinomas, that bexarotene inhibited both noninvasive and invasive adenocarcinomas, and that an improved effect on invasive adenocarcinomas was observed. Importantly, low-dose combination of raloxifene and bexarotene when administered during the promotion and progression stages (adenoma) resulted in significant inhibition of azoxymethane-induced colon adenocarcinomas in F344 rats. The anti-invasive activity of the raloxifene and bexarotene combination may have clinical application; thus, these drugs have potential for chemoprevention of colon cancer in high-risk individuals.

No potential conflicts of interest were disclosed.

Conception and design: N.B. Janakiram, A. Mohammed, V.E. Steele, C.V. Rao

Development of methodology: N.B. Janakiram, A. Mohammed, Y. Zhang, M. Brewer, T. Bryant, C.V. Rao

Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): N.B. Janakiram, A. Mohammed, Y. Zhang, M. Brewer, T. Bryant, S. Lightfoot, C.V. Rao

Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): N.B. Janakiram, S. Lightfoot, C.V. Rao

Writing, review, and/or revision of the manuscript: N.B. Janakiram, A. Mohammed, V.E. Steele, C.V. Rao

Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): C.V. Rao

Study supervision: C.V. Rao

The authors thank Dr. Sando for her editorial assistance with this article. They also thank the Rodent Barrier Facility staff for their support during the bioassay studies.

This investigation was supported by Grant NCI-N01 CN-53300 from the National Cancer Institute to C.V. Rao.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1.
Campbell-Thompson
M
,
Lynch
IJ
,
Bhardwaj
B
. 
Expression of estrogen receptor (ER) subtypes and ERbeta isoforms in colon cancer
.
Cancer Res
2001
;
61
:
632
40
.
2.
Jassam
N
,
Bell
SM
,
Speirs
V
,
Quirke
P
. 
Loss of expression of oestrogen receptor beta in colon cancer and its association with Dukes' staging
.
Oncol Rep
2005
;
14
:
17
21
.
3.
Janakiram
NB
,
Steele
VE
,
Rao
CV
. 
Estrogen receptor-beta as a potential target for colon cancer prevention: chemoprevention of azoxymethane-induced colon carcinogenesis by raloxifene in F344 rats
.
Cancer Prev Res
2009
;
2
:
52
9
.
4.
Thomas
ML
,
Xu
X
,
Norfleet
AM
,
Watson
CS
. 
The presence of functional estrogen receptors in intestinal epithelial cells
.
Endocrinology
1993
;
132
:
426
30
.
5.
Witte
D
,
Chirala
M
,
Younes
A
,
Li
Y
,
Younes
M
. 
Estrogen receptor β is expressed in human colorectal adenocarcinoma
.
Hum Pathol
2001
;
32
:
940
4
.
6.
Chlebowski
RT
,
Wactawski-Wende
J
,
Ritenbaugh
C
,
Hubbell
FA
,
Ascensao
J
,
Rodabough
RJ
, et al
Estrogen plus progestin and colorectal cancer in postmenopausal women
.
N Engl J Med
2004
;
350
:
991
1004
.
7.
Hulley
S
,
Furberg
C
,
Barrett-Connor
E
,
Grady
D
,
Haskell
W
,
Knopp
R
, et al
Noncardiovascular disease outcomes during 6.8 years of hormone therapy: Heart and Estrogen/Progestin Replacement Study follow-up (HERS II)
.
JAMA
2002
;
288
:
58
66
.
8.
Gunter
MJ
,
Hoover
DR
,
Yu
H
,
Wassertheil-Smoller
S
,
Rohan
TE
,
Manson
JE
, et al
Insulin, insulin-like growth factor-I, endogenous estradiol, and risk of colorectal cancer in postmenopausal women
.
Cancer Res
2008
;
68
:
329
37
.
9.
Clendenen
TV
,
Koenig
KL
,
Shore
RE
,
Levitz
M
,
Arslan
AA
,
Zeleniuch-Jacquotte
A
. 
Postmenopausal levels of endogenous sex hormones and risk of colorectal cancer
.
Cancer Epidemiol Biomarkers Prev
2009
;
18
:
275
81
.
10.
Wu
H
,
Xu
L
,
Chen
J
,
Hu
J
,
Yu
S
,
Hu
G
, et al
Association of estrogen receptor beta variants and serum levels of estradiol with risk of colorectal cancer: a case control study
.
BMC Cancer
2012
;
12
:
27
.
11.
Simon
MS
,
Chlebowski
RT
,
Wactawski-Wende
J
,
Johnson
KC
,
Muskovitz
A
,
Kato
I
, et al
Estrogen plus progestin and colorectal cancer incidence and mortality
.
J Clin Oncol
2012
;
30
:
3983
90
.
12.
Lonard
DM
,
Smith
CL
. 
Molecular perspectives on selective estrogen receptor modulators (SERMs): progress in understanding their tissuespecific agonist and antagonist actions
.
Steroid
2002
;
67
:
15
24
.
13.
Anzano
MA
,
Peer
CW
,
Smith
JM
,
Mullen
LT
,
Shrader
MW
,
Logsdon
DL
, et al
Chemoprevention of mammary carcinogenesis in the rat: combined use of raloxifene and 9-cis-retinoic acid
.
J Natl Cancer Inst
1996
;
88
:
123
5
.
14.
Janakiram
NB
,
Cooma
I
,
Mohammed
A
,
Steele
VE
,
Rao
CV
. 
B-Ionone inhibits colonic aberrant crypt foci formation in rats, suppresses cell growth, and induces retinoid X receptor-A in human colon cancer cells
.
Mol Cancer Ther
2008
;
7
:
181
9
.
15.
Janakiram
NB
,
Mohammed
A
,
Qian
L
,
Choi
C-I
,
Steele
VE
,
Rao
CV
. 
Chemopreventive effects of RXR-selective rexinoid bexarotene on intestinal neoplasia of ApcMin/+Mice
.
Neoplasia
2012
;
14
:
159
68
.
16.
Segars
JH
,
Marks
MS
,
Hirschfeld
S
,
Driggers
PH
,
Martinez
E
,
Grippo
JF
, et al
Inhibition of estrogen-responsive gene activation by the retinoid X receptor beta: evidence for multiple inhibitory pathways
.
Mol Cell Biol
1993
;
13
:
2258
68
.
17.
Suh
N
,
Lamph
WW
,
Glasebrook
AL
,
Grese
TA
,
Palkowitz
AD
,
Williams
CR
, et al
Prevention and treatment of experimental breast cancer with the combination of a new selective estrogen receptor modulator, arzoxifene, and a new rexinoid, LG 1002681
.
Clin Cancer Res
2002
;
8
:
3270
5
.
18.
Price
RH
 Jr
,
Lorenzon
N
,
Handa
RJ
. 
Differential expression of estrogen receptor beta splice variants in rat brain: identification and characterization of a novel variant missing exon 4
.
Mol Brain Res
2000
;
80
:
260
8
.
19.
Fiorelli
G
,
Picariello
L
,
Martineti
V
,
Tonelli
F
,
Brandi
ML
. 
Functional estrogen receptor beta in colon cancer cells
.
Biochem Biophys Res Commun
1999
;
261
:
521
7
.
20.
Xie
L-Q
,
Yu
J-P
,
Luo
H-S
. 
Expression of estrogen receptor b in human colorectal cancer
.
World J Gastroenterol
2004
;
10
:
214
7
.
21.
Konstantinopoulos
PA
,
Kominea
AV
,
Oros
G
,
Sykiotisa
GP
,
Andricopoulosd
P
,
Varakisb
I
, et al
Oestrogen receptor β (ERβ) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumour's dedifferentiation
.
Eur J Cancer
2003
;
39
:
1251
8
.
22.
Anderson
GL
,
Limacher
M
,
Assaf
AR
,
Bassford
T
,
Beresford
SA
,
Black
H
, et al
Effects of conjugated equine estrogen in postmenopausal women with hysterectomy: the Women's Health Initiative randomized controlled trial
.
JAMA
2004
;
291
:
1701
12
.
23.
Ritenbaugh
C
,
Stanford
JL
,
Wu
L
,
Shikany
JM
,
Schoen
RE
,
Stefanick
ML
, et al
Conjugated equine estrogens and colorectal cancer incidence and survival: the Women's Health Initiative randomized clinical trial
.
Cancer Epidemiol Biomarkers Prev
2008
;
17
:
2609
18
.
24.
Saleiro
D
,
Murillo
G
,
Benya
RV
,
Bissonnette
M
,
Hart
J
,
Mehta
RG
. 
Estrogen receptor-beta protects against colitis-associated neoplasia in mice
.
Int J Cancer
2012
;
131
:
2553
61
.
25.
Giroux
V
,
Lemay
F
,
Bernatchez
G
,
Robitaille
Y
,
Carrier
JC
. 
Estrogen receptor β deficiency enhances small intestinal tumorigenesis in ApcMin/+mice
.
Int J Cancer
2008
;
123
:
303
11
.
26.
Giroux
V
,
Bernatchez
G
,
Carrier
JC
. 
Chemopreventive effect of ERβ-selective agonist on intestinal tumorigenesis in Apc(Min/+) mice
.
Mol Carcinog
2011
;
50
:
359
69
.
27.
Heijmans
J
,
Wielenga
MC
,
Rosekrans
SL
,
van Lidth de Jeude
JF
,
Roelofs
J
,
Groothuis
P
, et al
Oestrogens promote tumorigenesis in a mouse model for colitis-associated cancer
.
Gut
2013 Feb 13
.
[Epub ahead of print]
.
28.
Janakiram
NB
,
Mohammed
A
,
Qian
L
,
Venkateshwar
M
,
Reddy
MP
,
Steele
VE
, et al
Chemopreventive effects of selective estrogen receptor modulator (SERM) raloxifene on intestinal neoplasia of APCMin/+ mice
. In:
Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2–6; Orlando, FL
.
Philadelphia (PA)
:
AACR
; 
2011
;
71
(
8 Suppl
).
Abstract nr 831
.
29.
Vogel
VG
. 
The NSABP Study of Tamoxifen and Raloxifene (STAR) trial
.
Expert Rev Anticancer Ther
2009
;
9
:
51
60
.
30.
Martínez
ME
,
Baron
JA
,
Lieberman
DA
,
Schatzkin
A
,
Lanza
E
,
Winawer
SJ
, et al
A pooled analysis of advanced colorectal neoplasia diagnoses after colonoscopic polypectomy
.
Gastroenterology
2009
;
136
:
832
41
.
31.
Cummings
SR
,
Eckert
S
,
Krueger
KA
,
Grady
D
,
Powles
TJ
,
Cauley
JA
, et al
The effect of raloxifene on risk of breast cancer in postmenopausal women: results from the MORE randomized trial. Multiple Outcomes of Raloxifene Evaluation
.
JAMA
1999
;
281
:
2189
97
.
32.
Barrett-Connor
E
,
Mosca
L
,
Collins
P
,
Geiger
MJ
,
Grady
D
,
Kornitzer
M
, et al
Effects of raloxifene on cardiovascular events and breast cancer in postmenopausal women
.
N Engl J Med
2006
;
355
:
125
37
.
33.
Walsh
JM
,
Cheung
AM
,
Yang
D
,
Litwack
S
,
Grady
D
. 
J. Raloxifene and colorectal cancer
.
Womens Health (Larchmt)
2005
;
14
:
299
305
.
34.
Wang
Y
,
Zhang
Z
,
Yao
R
,
Jia
D
,
Wang
D
,
Lubet
RA
, et al
Prevention of lung cancer progression by bexarotene in mouse models
.
Oncogene
2006
;
25
:
1320
9
.
35.
Zanardi
S
,
Serrano
D
,
Argusti
A
,
Barile
M
,
Puntoni
M
,
Decensi
A
. 
Clinical trials with retinoids for breast cancer chemoprevention
.
Endocr Relat Cancer
2006
;
13
:
51
68
.
36.
Wu
K
,
Kim
HT
,
Rodriquez
JL
,
Hilsenbeck
SG
,
Mohsin
SK
,
Xu
XC
, et al
Suppression of mammary tumorigenesis in transgenic mice by the RXR-selective retinoid, LGD1069
.
Cancer Epidemiol Biomarkers Prev
2002
;
11
:
467
74
.
37.
Gottardis
MM
,
Bischoff
ED
,
Shirley
MA
,
Wagoner
MA
,
Lamph
WW
,
Heyman
RA
. 
Chemoprevention of mammary carcinoma by LGD1069 (Targretin): an RXR-selective ligand
.
Cancer Res
1996
;
56
:
5566
70
.
38.
Wu
K
,
Zhang
Y
,
Xu
XC
,
Hill
J
,
Celestino
J
,
Kim
HT
, et al
The retinoid X receptor-selective retinoid, LGD1069, prevents the development of estrogen receptor-negative mammary tumors in transgenic mice
.
Cancer Res
2002
;
62
:
6376
80
.
39.
Bischoff
ED
,
Gottardis
MM
,
Moon
TE
,
Heyman
RA
,
Lamph
WW
. 
Beyond tamoxifen: the retinoid X receptor-selective ligand LGD1069 (TARGRETIN) causes complete regression of mammary carcinoma
.
Cancer Res
1998
;
58
:
479
84
.
40.
Cohen
SM
. 
Role of cell proliferation in regenerative and neoplastic disease
.
Toxicol Lett
1995
;
82–83
:
15
21
.
41.
Williams
GT
. 
Programmed cell death: apoptosis and oncogenesis
.
Cell
1991
;
65
:
1097
8
.
42.
Sparks
AB
,
Morin
PJ
,
Vogelstein
B
,
Kinzler
KW
. 
Mutational analysis of the APC/beta-catenin/Tcf pathway in colorectal cancer
.
Cancer Res
1998
;
58
:
1130
4
.
43.
Zanardi
S
,
Serrano
D
,
Argusti
A
,
Barile
M
,
Puntoni
M
,
Decensi
A
. 
Clinical trials with retinoids for breast cancer chemoprevention
.
Endocr Relat Cancer
2006
;
13
:
51
68
.
44.
Kumru
S
,
Yildiz
FM
,
Godekmerdan
A
,
Kutlu
S
,
Yilmaz
B
,
Gurates
B
. 
Effects of raloxifene and hormone replacement therapy on serum Th2 and Th3 type cytokine concentrations in healthy postmenopausal women: a randomised controlled trial
.
Arch Gynecol Obstet
2008
;
277
:
489
93
.
45.
Budgin
JB
,
Richardson
SK
,
Newton
SB
,
Wysocka
M
,
Zaki
MH
,
Benoit
B
, et al
Biological effects of bexarotene in cutaneous T-cell lymphoma
.
Arch Dermatol
2005
;
141
:
315
20
.
46.
Walsh
BW
,
Cox
DA
,
Sashegyi
A
,
Dean
RA
,
Tracy
RP
,
Anderson
PW
. 
Role of tumor necrosis factor- and interleukin-6 in the effects of hormone replacement therapy and raloxifene on C-reactive protein in postmenopausal women
.
Am J Cardiol
2001
;
88
:
825
8
.
47.
Gianni
W
,
Ricci
A
,
Gazzaniga
P
,
Brama
M
,
Pietropaolo
M
,
Votano
S
, et al
Raloxifene modulates interleukin-6 and tumor necrosis factor-alpha synthesis in vivo: results from a pilot clinical study
.
J Clin Endocrinol Metab
2004
;
89
:
6079
99
.
48.
Ozmen
B
,
Kirmaz
C
,
Aydin
K
,
Kafescller
SO
,
Guclu
F
,
Hekimsoy
Z
. 
Influence of the selective oestrogen receptor modulator (raloxifene hydrochloride) on IL-6, TNF-α, TGF-β1 and bone turnover markers in the treatment of postmenopausal osteoporosis
.
Eur Cytokine Netw
2007
;
18
:
148
53
.
49.
Yasui
T
,
Uemura
H
,
Hyodo
S
,
Yamada
M
,
Yamamoto
S
,
Maegawa
M
, et al
Raloxifene reduces circulating levels of interleukin-7 and monocyte chemoattractant protein-1 in postmenopausal women
.
Atherosclerosis
2009
;
204
:
471
5
.